Detailed information of TA system TA124327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4464862..4465556
• Replicon: chromosome
• Accession: NZ_CP039834
• Organism: Escherichia coli O157:H7 strain MB41-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FCV42_RS22590
• Protein ID: WP_001263495.1
• Coordinates: 4464862..4465260 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FCV42_RS22595
• Protein ID: WP_000554757.1
• Coordinates: 4465263..4465556 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4460449..4460529	-	81	NuclAT_12	-	-
-	4460449..4460529	-	81	NuclAT_12	-	-
-	4460449..4460529	-	81	NuclAT_12	-	-
-	4460449..4460529	-	81	NuclAT_12	-	-
FCV42_RS22565	4461125..4461583	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FCV42_RS22570	4461844..4463301	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FCV42_RS22575	4463358..4463915	-	558	WP_023438539.1	peptide chain release factor H	-
FCV42_RS22580	4463827..4464093	-	267	WP_001303804.1	hypothetical protein	-
FCV42_RS22585	4464400..4464852	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FCV42_RS22590	4464862..4465260	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FCV42_RS22595	4465263..4465556	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FCV42_RS22600	4465608..4466663	-	1056	WP_001226186.1	DNA polymerase IV	-
FCV42_RS22605	4466734..4467519	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FCV42_RS22610	4467464..4469203	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
FCV42_RS22615	4469348..4469845	-	498	Protein_4429	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T124327 WP_001263495.1 NZ_CP039834:c4465260-4464862 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T124327 NZ_CP039834:c4465260-4464862 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT124327 WP_000554757.1 NZ_CP039834:c4465556-4465263 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT124327 NZ_CP039834:c4465556-4465263 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6