Detailed information of TA system TA124277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 103489..104216
• Replicon: chromosome
• Accession: NZ_CP039819
• Organism: Klebsiella pneumoniae strain C2414

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: FA958_RS00495
• Protein ID: WP_004118600.1
• Coordinates: 103489..103800 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: FA958_RS00500
• Protein ID: WP_000990392.1
• Coordinates: 103797..104216 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA958_RS00475	100003..100722	+	720	WP_001102107.1	(Fe-S)-binding protein	-
FA958_RS00480	100733..102160	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
FA958_RS00485	102153..102848	+	696	WP_004118595.1	lactate utilization protein C	-
FA958_RS00490	102898..103278	-	381	Protein_96	gluconate permease	-
FA958_RS00495	103489..103800	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
FA958_RS00500	103797..104216	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
FA958_RS00505	104253..105455	-	1203	WP_003031541.1	hypothetical protein	-
FA958_RS00510	105448..105777	-	330	WP_004118613.1	hypothetical protein	-
FA958_RS00515	105774..106433	-	660	WP_000078540.1	hypothetical protein	-
FA958_RS29825	106857..107450	-	594	WP_044348674.1	hypothetical protein	-
FA958_RS00525	107766..108119	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FA958_RS00530	108167..108529	+	363	WP_020324593.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(A) / qacE / sul1 / mph(A) / aph(3')-Ia	-	80702..156761	76059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T124277 WP_004118600.1 NZ_CP039819:103489-103800 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T124277 NZ_CP039819:103489-103800 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT124277 WP_000990392.1 NZ_CP039819:103797-104216 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT124277 NZ_CP039819:103797-104216 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4