Detailed information of TA system TA124275

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 152460..152713
• Replicon: plasmid pC2660-3-KPC
• Accession: NZ_CP039810
• Organism: Klebsiella pneumoniae strain C2660

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FA957_RS29855
• Protein ID: WP_001312851.1
• Coordinates: 152564..152713 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	152460..152519 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA957_RS29815	148120..148185	-	66	Protein_187	helix-turn-helix domain-containing protein	-
FA957_RS29820	148238..148942	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FA957_RS29825	149006..149167	+	162	Protein_189	DNA helicase	-
FA957_RS29830	149187..149933	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FA957_RS29835	149988..150548	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FA957_RS29840	150680..150880	+	201	WP_015059022.1	hypothetical protein	-
FA957_RS29845	151266..151865	+	600	WP_032083981.1	hypothetical protein	-
FA957_RS29850	152029..152259	+	231	WP_001736714.1	hypothetical protein	-
-	152460..152519	-	60	NuclAT_1	-	Antitoxin
-	152460..152519	-	60	NuclAT_1	-	Antitoxin
-	152460..152519	-	60	NuclAT_1	-	Antitoxin
-	152460..152519	-	60	NuclAT_1	-	Antitoxin
FA957_RS29855	152564..152713	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
FA957_RS29860	152997..153245	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..153556	153556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T124275 WP_001312851.1 NZ_CP039810:152564-152713 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T124275 NZ_CP039810:152564-152713 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT124275 NZ_CP039810:c152519-152460 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin