Detailed information of TA system TA124270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39467..39737
• Replicon: plasmid pC2660-3-KPC
• Accession: NZ_CP039810
• Organism: Klebsiella pneumoniae strain C2660

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FA957_RS29040
• Protein ID: WP_001312861.1
• Coordinates: 39579..39737 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39467..39530 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA957_RS29015	35178..35705	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FA957_RS29020	35763..35996	+	234	WP_000006003.1	DUF905 family protein	-
FA957_RS29025	36057..38080	+	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
FA957_RS29030	38149..38583	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FA957_RS29035	38580..39299	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	39311..39535	+	225	NuclAT_0	-	-
-	39311..39535	+	225	NuclAT_0	-	-
-	39311..39535	+	225	NuclAT_0	-	-
-	39311..39535	+	225	NuclAT_0	-	-
-	39467..39530	-	64	-	-	Antitoxin
FA957_RS29040	39579..39737	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FA957_RS30475	39975..40352	-	378	Protein_50	hypothetical protein	-
FA957_RS29050	40652..40948	+	297	WP_001272251.1	hypothetical protein	-
FA957_RS29055	41059..41880	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FA957_RS29060	42177..42824	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FA957_RS29065	43101..43484	+	384	WP_000124981.1	relaxosome protein TraM	-
FA957_RS29070	43675..44361	+	687	WP_015059009.1	PAS domain-containing protein	-
FA957_RS29075	44455..44682	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..153556	153556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T124270 WP_001312861.1 NZ_CP039810:39579-39737 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T124270 NZ_CP039810:39579-39737 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT124270 NZ_CP039810:c39530-39467 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin