Detailed information of TA system TA124212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3060194..3060894
• Replicon: chromosome
• Accession: NZ_CP039700
• Organism: Vibrio cyclitrophicus strain ECSMB14105

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: FAZ90_RS13845
• Protein ID: WP_197080429.1
• Coordinates: 3060194..3060604 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: FAZ90_RS13850
• Protein ID: WP_046209475.1
• Coordinates: 3060601..3060894 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FAZ90_RS13820	3055868..3056350	+	483	WP_016792660.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
FAZ90_RS13830	3056663..3056986	-	324	WP_016792662.1	nucleotidyltransferase domain-containing protein	-
FAZ90_RS13835	3056976..3057440	-	465	WP_016792663.1	HI0074 family nucleotidyltransferase substrate-binding subunit	-
FAZ90_RS13840	3057748..3059280	-	1533	WP_016790150.1	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	-
FAZ90_RS13845	3060194..3060604	-	411	WP_197080429.1	type II toxin-antitoxin system YafO family toxin	Toxin
FAZ90_RS13850	3060601..3060894	-	294	WP_046209475.1	hypothetical protein	Antitoxin
FAZ90_RS13855	3061062..3061250	-	189	WP_136952235.1	hypothetical protein	-
FAZ90_RS13860	3061923..3062966	-	1044	WP_046209476.1	alkene reductase	-
FAZ90_RS13865	3063259..3063948	-	690	WP_046209477.1	hypothetical protein	-
FAZ90_RS13870	3063969..3065570	-	1602	WP_052714397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	htpB / ugd / wbtL	3039977..3113344	73367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (33-134)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15787.00 Da
• Isoelectric Point: 7.1715

>T124212 WP_197080429.1 NZ_CP039700:c3060604-3060194 [Vibrio cyclitrophicus]
VSKSLNIRVFKHKILIDALTESELHSLTKDFRYYKETGQKPEFFGRDEAYDHPNTLPILKSEEVKHIHLAATDAPFLSSI
QFYQTSDKHLVYCQGWNEPSCFLLIAILAPDAHEQARNRTIMHNLGLIAEKFRQNF

Nucleotide

• Length: 411 bp

>T124212 NZ_CP039700:c3060604-3060194 [Vibrio cyclitrophicus]
GTGAGTAAGTCATTAAATATTCGAGTTTTTAAGCATAAAATCTTAATTGATGCACTTACTGAAAGTGAGCTTCATTCTTT
GACTAAAGATTTTAGGTACTATAAGGAGACTGGTCAAAAACCAGAATTCTTTGGTCGTGATGAAGCTTATGATCATCCCA
ATACATTACCAATCCTGAAGTCAGAAGAAGTGAAGCACATTCACTTAGCTGCTACTGATGCCCCATTCTTAAGTTCTATC
CAGTTTTACCAAACAAGTGATAAGCACTTAGTGTATTGCCAAGGCTGGAATGAACCGAGCTGTTTCCTGTTAATTGCGAT
CTTAGCACCTGATGCTCATGAGCAAGCTCGTAATAGAACTATCATGCATAACTTGGGTTTGATTGCTGAAAAATTTAGGC
AAAATTTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10603.00 Da
• Isoelectric Point: 4.6362

>AT124212 WP_046209475.1 NZ_CP039700:c3060894-3060601 [Vibrio cyclitrophicus]
MKTSTIYAEKAVSSSEARKHFKDCLSGEPVVVLSNNEPAGYMMSATFFEGLMKALEGKNADSASLFRPMSARLEVIAHLN
AQALTELDENTLGEFQE

Nucleotide

• Length: 294 bp

>AT124212 NZ_CP039700:c3060894-3060601 [Vibrio cyclitrophicus]
ATGAAAACAAGTACAATTTATGCAGAAAAAGCAGTGTCATCAAGCGAAGCTAGAAAGCACTTTAAAGATTGCTTATCAGG
GGAGCCTGTCGTCGTTTTATCGAACAATGAGCCTGCTGGTTATATGATGAGTGCAACTTTTTTTGAAGGACTTATGAAAG
CTCTAGAAGGCAAGAATGCTGACTCTGCTTCATTATTTAGACCAATGAGTGCTCGCTTAGAAGTCATTGCCCACCTGAAT
GCTCAAGCATTAACAGAACTTGATGAGAATACTTTGGGTGAGTTTCAAGAGTGA