Detailed information of TA system TA124189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 862817..863511
• Replicon: chromosome
• Accession: NZ_CP039650
• Organism: Azospirillum sp. TSA2s

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E6C67_RS26155
• Protein ID: WP_136704623.1
• Coordinates: 862817..863176 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6C67_RS26160
• Protein ID: WP_136704624.1
• Coordinates: 863173..863511 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6C67_RS26135	858923..859192	+	270	WP_211103617.1	hypothetical protein	-
E6C67_RS26140	859817..861169	+	1353	WP_136701028.1	IS66 family transposase	-
E6C67_RS26145	861235..862587	+	1353	WP_136701028.1	IS66 family transposase	-
E6C67_RS26155	862817..863176	+	360	WP_136704623.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6C67_RS26160	863173..863511	+	339	WP_136704624.1	XRE family transcriptional regulator	Antitoxin
E6C67_RS26165	863663..866356	-	2694	WP_136704625.1	pyruvate, phosphate dikinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12944.79 Da
• Isoelectric Point: 10.7258

>T124189 WP_136704623.1 NZ_CP039650:862817-863176 [Azospirillum sp. TSA2s]
MHTVLFHTAFAAEFRALPRAVRIDLAAAAKVLGERGPALGRPHVDTLNGSRHANMKELRFKSNGGVWRVAFAFDPTRNAV
VLVAGDKSGVSEKRFYASLIATADSRYDDHLSDLKGAKP

Nucleotide

• Length: 360 bp

>T124189 NZ_CP039650:862817-863176 [Azospirillum sp. TSA2s]
ATGCACACCGTTCTCTTCCACACCGCGTTCGCGGCCGAATTCCGGGCTCTGCCGCGAGCCGTGCGGATAGACCTCGCCGC
CGCGGCCAAGGTGCTTGGGGAGCGTGGCCCGGCGCTCGGCCGGCCCCATGTCGATACATTGAACGGTTCCCGGCATGCGA
ACATGAAGGAGTTGCGGTTCAAATCGAATGGCGGGGTGTGGCGCGTCGCCTTTGCCTTCGACCCGACGCGCAACGCCGTC
GTGCTGGTTGCCGGCGACAAGAGCGGGGTTTCGGAAAAGCGGTTCTACGCCTCGCTGATCGCCACCGCCGACAGCCGCTA
TGACGACCATCTCTCCGACCTGAAAGGAGCAAAGCCATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12153.86 Da
• Isoelectric Point: 4.9167

>AT124189 WP_136704624.1 NZ_CP039650:863173-863511 [Azospirillum sp. TSA2s]
MTATIPFDQILSEMTDDERAAVDARAAGMLAEIDGLAELRRLAELTQGQVAETLNVKQPTVHQIEKRADLYLSTLRRFVE
AAGGELELRVSLPGKGAFKLTGIGELTSGSRR

Nucleotide

• Length: 339 bp

>AT124189 NZ_CP039650:863173-863511 [Azospirillum sp. TSA2s]
ATGACGGCGACGATCCCGTTCGACCAGATCCTCTCTGAGATGACCGACGACGAGCGCGCCGCGGTGGATGCACGGGCCGC
CGGGATGCTGGCCGAGATCGACGGATTGGCGGAGCTGCGCCGGCTGGCGGAACTGACCCAGGGTCAGGTTGCCGAAACCC
TGAACGTGAAGCAGCCGACCGTCCACCAGATCGAGAAGCGGGCCGACCTGTATCTGTCCACCCTGCGGCGCTTCGTGGAA
GCCGCGGGCGGGGAGTTGGAATTGCGGGTTTCCCTTCCGGGAAAAGGGGCGTTCAAGCTGACGGGCATCGGCGAGTTGAC
CTCCGGCTCCCGGCGCTGA