Detailed information of TA system TA124177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 422977..423569
• Replicon: chromosome
• Accession: NZ_CP039631
• Organism: Pseudomonas veronii strain Pvy

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: E4167_RS02015
• Protein ID: WP_081610097.1
• Coordinates: 423291..423569 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: A0A1D3JSM0
• Locus tag: E4167_RS02010
• Protein ID: WP_017844583.1
• Coordinates: 422977..423282 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4167_RS01995	418697..419683	+	987	WP_046485807.1	c-type cytochrome	-
E4167_RS02000	419694..421508	+	1815	WP_046485809.1	GHKL domain-containing protein	-
E4167_RS02005	421505..422896	+	1392	WP_155677195.1	response regulator	-
E4167_RS02010	422977..423282	-	306	WP_017844583.1	HigA family addiction module antidote protein	Antitoxin
E4167_RS02015	423291..423569	-	279	WP_081610097.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4167_RS02020	423873..425495	+	1623	WP_155677196.1	FMN-binding glutamate synthase family protein	-
E4167_RS02025	425508..425792	+	285	WP_017844581.1	antibiotic biosynthesis monooxygenase	-
E4167_RS02030	425849..426028	-	180	WP_141122991.1	hypothetical protein	-
E4167_RS02035	426060..427208	+	1149	WP_046485814.1	OpgC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10538.01 Da
• Isoelectric Point: 7.2444

>T124177 WP_081610097.1 NZ_CP039631:c423569-423291 [Pseudomonas veronii]
MIRSFKCAETPKLFETGRTRRWSAVVSVVERQLSMLDAAVELADLRSPPGNRLEPLYGDREGQHSIRINGQFRVCFIWGP
HGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T124177 NZ_CP039631:c423569-423291 [Pseudomonas veronii]
ATGATCAGAAGCTTTAAATGCGCCGAAACCCCAAAACTGTTTGAAACCGGTCGAACCCGACGTTGGTCCGCTGTCGTTTC
CGTGGTGGAACGACAGCTGAGCATGCTGGATGCGGCGGTTGAACTCGCTGATTTGCGCTCACCGCCCGGGAATAGGCTGG
AACCGTTATATGGTGACAGAGAGGGGCAACACAGTATCCGAATCAATGGTCAATTCCGGGTCTGTTTCATTTGGGGGCCT
CACGGCCCTGAAAATGTTGAAATTGTGGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11115.93 Da
• Isoelectric Point: 7.2767

>AT124177 WP_017844583.1 NZ_CP039631:c423282-422977 [Pseudomonas veronii]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSISADVALRLAVALETSAQFWLNLQTAYDLR
TAEIAKGAQIYKEVERVAHCA

Nucleotide

• Length: 306 bp

>AT124177 NZ_CP039631:c423282-422977 [Pseudomonas veronii]
ATGTTTAAGAATGGCATGCGACCTGTACATCCTGGGGAAATACTCAAGGAAGAGTACCTGGAGCCGCTGGGCCTGACCGC
AGCCGCTCTCGCAAGGGCGCTGCATGTTTCCGCCCCTACGGTGAATGACATTGTATTGATGCGTCGGAGCATCAGTGCGG
ACGTTGCATTACGCCTGGCAGTCGCGTTGGAGACATCTGCACAGTTCTGGCTGAACCTGCAGACCGCCTATGATCTGCGC
ACTGCGGAAATTGCCAAAGGCGCCCAGATTTACAAAGAAGTCGAGAGAGTTGCGCATTGCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1D3JSM0