Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 456064..456666 Replicon chromosome
Accession NZ_CP039453
Organism Enterobacter bugandensis strain 220

Toxin (Protein)


Gene name higB Uniprot ID A0A167J774
Locus tag FBF69_RS02195 Protein ID WP_047366784.1
Coordinates 456064..456375 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A167J796
Locus tag FBF69_RS02200 Protein ID WP_047366785.1
Coordinates 456376..456666 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FBF69_RS02165 451723..452466 + 744 WP_048959977.1 winged helix-turn-helix domain-containing protein -
FBF69_RS02170 452463..452933 + 471 WP_136484716.1 hypothetical protein -
FBF69_RS02175 453021..453620 + 600 WP_045260234.1 glucose-1-phosphatase -
FBF69_RS02180 453614..454495 + 882 WP_045260235.1 virulence factor BrkB family protein -
FBF69_RS02185 454492..454929 + 438 WP_008501836.1 D-tyrosyl-tRNA(Tyr) deacylase -
FBF69_RS02190 454974..455915 + 942 WP_028014875.1 fatty acid biosynthesis protein FabY -
FBF69_RS02195 456064..456375 + 312 WP_047366784.1 hypothetical protein Toxin
FBF69_RS02200 456376..456666 + 291 WP_047366785.1 helix-turn-helix domain-containing protein Antitoxin
FBF69_RS02205 456708..457637 - 930 WP_028014872.1 formate dehydrogenase accessory protein FdhE -
FBF69_RS02210 457634..458269 - 636 WP_015569914.1 formate dehydrogenase cytochrome b556 subunit -
FBF69_RS02215 458266..459168 - 903 WP_003861960.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12135.13 Da        Isoelectric Point: 8.8415

>T123987 WP_047366784.1 NZ_CP039453:456064-456375 [Enterobacter bugandensis]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPEYGDVIQNTGGLRKIRWLAGGKGKRGGVRVIYFYRTCEFEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Download         Length: 312 bp

>T123987 NZ_CP039453:456064-456375 [Enterobacter bugandensis]
ATGCTATTCATTGAAACGGACATTTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATCGGTTCCAGAT
ATTCCTGGCGACACAACCGGAGTACGGCGATGTTATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GAAAGGGAAAGCGTGGTGGAGTCAGAGTTATCTATTTTTACCGTACGTGTGAGTTTGAAATCAGACTGCTGCTCATCTAT
CGCAAAGGGATTAAAGATGACCTGTCTGCGGGGGAGAAAGCTATTCTGAAAAAGATGATAGAGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10674.22 Da        Isoelectric Point: 10.5888

>AT123987 WP_047366785.1 NZ_CP039453:456376-456666 [Enterobacter bugandensis]
MDKALFERLTQSVGQMNEIAEGQREPSRIFQIDAMKIKEIRQASGLSQSKFATLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Download         Length: 291 bp

>AT123987 NZ_CP039453:456376-456666 [Enterobacter bugandensis]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCGTTGGGCAAATGAACGAGATTGCCGAGGGCCAACGCGAGCCGTC
GCGTATCTTTCAAATCGATGCGATGAAAATAAAAGAAATTCGTCAGGCATCCGGGTTGTCGCAGAGTAAATTCGCGACCT
TAATCTCTGTCAGCGTTGATACATTGCGTAACTGGGAACAAGGCAGACGCTCGCCTACAGGCCCGGCTAAAGCGCTTTTG
CGCGCTATTGCTAACGATCCTCAACATGTCATCCCTGCGCTATCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167J774

Antitoxin

Source ID Structure
AlphaFold DB A0A167J796

References