Detailed information of TA system TA123986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 331572..332302
• Replicon: chromosome
• Accession: NZ_CP039453
• Organism: Enterobacter bugandensis strain 220

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FBF69_RS01615
• Protein ID: WP_048961381.1
• Coordinates: 331988..332302 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FBF69_RS01610
• Protein ID: WP_127334638.1
• Coordinates: 331572..331991 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF69_RS01585	327063..328568	-	1506	WP_063437534.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
FBF69_RS01590	328565..329158	-	594	WP_022646555.1	transcriptional regulator UhpA	-
FBF69_RS01595	329241..329528	-	288	WP_028014960.1	acetolactate synthase small subunit	-
FBF69_RS01600	329532..331220	-	1689	WP_045260834.1	acetolactate synthase large subunit	-
FBF69_RS01605	331327..331425	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
FBF69_RS01610	331572..331991	-	420	WP_127334638.1	helix-turn-helix domain-containing protein	Antitoxin
FBF69_RS01615	331988..332302	-	315	WP_048961381.1	type II toxin-antitoxin system HigB family toxin	Toxin
FBF69_RS01620	332790..332879	+	90	WP_045260836.1	type I toxin-antitoxin system toxin TisB	-
FBF69_RS01625	332944..333390	+	447	WP_028014956.1	GNAT family N-acetyltransferase	-
FBF69_RS01630	333453..334286	+	834	WP_136484674.1	DMT family transporter	-
FBF69_RS01635	334462..335646	+	1185	WP_049137210.1	multidrug efflux MFS transporter EmrD	-
FBF69_RS01640	335549..336637	-	1089	WP_136484676.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12402.51 Da
• Isoelectric Point: 10.2219

>T123986 WP_048961381.1 NZ_CP039453:c332302-331988 [Enterobacter bugandensis]
MHLISMKAIIDAVKQFPQHKEELLFLGRTIEKSHCPTPAALKQIFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T123986 NZ_CP039453:c332302-331988 [Enterobacter bugandensis]
ATGCATTTAATTTCTATGAAGGCAATTATTGATGCGGTAAAACAGTTCCCGCAGCACAAGGAAGAGCTCCTTTTTTTAGG
TCGCACTATCGAAAAGAGCCATTGCCCGACACCTGCTGCGCTGAAACAAATTTTTCCGACGCTGGATAACTTTAAATATC
TGGATAAGCATTATGTGATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTC
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15578.16 Da
• Isoelectric Point: 4.9534

>AT123986 WP_127334638.1 NZ_CP039453:c331991-331572 [Enterobacter bugandensis]
MMIVADAMKATHALVEAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARISRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT123986 NZ_CP039453:c331991-331572 [Enterobacter bugandensis]
ATGATGATCGTCGCTGATGCAATGAAAGCAACGCATGCTCTTGTCGAGGCTGTCCCTTTACTGGGTGAACATCCGAATGA
GAAAGATTACCAGGATGCACTTGAGCTGGTTGAGTATCTGCTCATGAATGAACCTGGTAGTCCTTTGCTGGATATCGTAT
GTGCAAGAATAAGTCGTTATGAAGCGAATCGACCCGAGATTGTCGCCTTGCGTCAGGAGATGGAATCTGTTCCAGTGGGA
ATTGCGGTTTTAAGAACCCTTATGGATCAGTACAAACTGACCATTTCAGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTATTGAATGGACAGCGTCAGCTGACGTTAAACCATATAAAAAAACTGGCGGTGCGCTTTGGGGTATCGC
CTGCACTGTTTATTGAATGA