Detailed information of TA system TA123976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2446666..2447323
• Replicon: chromosome
• Accession: NZ_CP039452
• Organism: Enterobacter bugandensis strain 1367

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FBF68_RS11920
• Protein ID: WP_048957972.1
• Coordinates: 2447024..2447323 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A822X4T6
• Locus tag: FBF68_RS11915
• Protein ID: WP_063155569.1
• Coordinates: 2446666..2447013 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF68_RS11885	2441853..2442587	+	735	WP_063155564.1	class I SAM-dependent methyltransferase	-
FBF68_RS11890	2442581..2443183	-	603	WP_028014155.1	short chain dehydrogenase	-
FBF68_RS11895	2443281..2444168	+	888	WP_063155565.1	LysR family transcriptional regulator	-
FBF68_RS11900	2444189..2445370	+	1182	WP_063155566.1	PLP-dependent aminotransferase family protein	-
FBF68_RS11905	2445416..2445835	-	420	WP_063155567.1	nuclear transport factor 2 family protein	-
FBF68_RS11910	2445933..2446664	+	732	WP_063155568.1	helix-turn-helix transcriptional regulator	-
FBF68_RS11915	2446666..2447013	-	348	WP_063155569.1	HigA family addiction module antidote protein	Antitoxin
FBF68_RS11920	2447024..2447323	-	300	WP_048957972.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FBF68_RS11925	2447639..2448808	-	1170	WP_088204896.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FBF68_RS11930	2448819..2451014	-	2196	WP_028014148.1	type I secretion system permease/ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11538.15 Da
• Isoelectric Point: 10.0104

>T123976 WP_048957972.1 NZ_CP039452:c2447323-2447024 [Enterobacter bugandensis]
MINHFRDQWLKDFFLYGKSSNVIPSSLESALARKLDMINAATSHRELRSPPGNMYESLNPPLAGYSAIRVNRQYRLVFRW
SEGKADDLYLSPHKYTPYK

Nucleotide

• Length: 300 bp

>T123976 NZ_CP039452:c2447323-2447024 [Enterobacter bugandensis]
ATGATTAATCATTTTCGTGATCAATGGCTTAAGGATTTTTTTCTGTACGGGAAATCAAGCAATGTGATCCCTTCAAGCCT
TGAATCAGCCTTAGCGAGGAAGCTGGATATGATCAACGCTGCCACCTCGCACCGGGAGCTGAGATCCCCGCCAGGCAATA
TGTATGAATCGCTGAATCCCCCGCTTGCAGGGTACTCTGCGATTCGCGTTAACAGGCAATACAGGTTGGTATTCAGGTGG
TCAGAGGGCAAAGCAGACGATCTCTACCTCTCTCCGCACAAGTACACGCCATACAAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13188.19 Da
• Isoelectric Point: 8.5279

>AT123976 WP_063155569.1 NZ_CP039452:c2447013-2446666 [Enterobacter bugandensis]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAFRLAKAFDTTIEFWLKLQLNVD
IWEAQTNSRTQEELSRIKTIAEIMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT123976 NZ_CP039452:c2447013-2446666 [Enterobacter bugandensis]
ATGACTCTTCAACAGGCTCTACGCAAACCCACCACGCCGGGCGATGTGTTGCAGTATGAATATCTTGAGCCGCTCAATCT
GAAAATTAGCGATCTCGCGGAAATGCTTAACGTGCACCGTAACACCATCAGTGCTCTCGTTAATAATAATCGTAAACTGA
CGGCCGATATGGCATTCAGACTGGCCAAGGCGTTTGATACCACTATTGAATTCTGGTTGAAACTGCAGCTAAACGTCGAC
ATCTGGGAAGCACAAACTAATTCCCGGACTCAGGAGGAGTTGAGCCGTATAAAAACGATTGCAGAAATCATGGCAAAAAG
GAAATCAGGCCAGCCGGATGTGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A822X4T6