Detailed information of TA system TA123973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1344987..1345717
• Replicon: chromosome
• Accession: NZ_CP039452
• Organism: Enterobacter bugandensis strain 1367

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A822X036
• Locus tag: FBF68_RS06505
• Protein ID: WP_063155234.1
• Coordinates: 1345403..1345717 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FBF68_RS06500
• Protein ID: WP_127334638.1
• Coordinates: 1344987..1345406 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF68_RS06475	1340478..1341983	-	1506	WP_063155233.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
FBF68_RS06480	1341980..1342573	-	594	WP_022646555.1	transcriptional regulator UhpA	-
FBF68_RS06485	1342656..1342943	-	288	WP_028014960.1	acetolactate synthase small subunit	-
FBF68_RS06490	1342947..1344635	-	1689	WP_059353827.1	acetolactate synthase large subunit	-
FBF68_RS06495	1344742..1344840	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
FBF68_RS06500	1344987..1345406	-	420	WP_127334638.1	helix-turn-helix domain-containing protein	Antitoxin
FBF68_RS06505	1345403..1345717	-	315	WP_063155234.1	type II toxin-antitoxin system HigB family toxin	Toxin
FBF68_RS06510	1346205..1346294	+	90	WP_045260836.1	type I toxin-antitoxin system toxin TisB	-
FBF68_RS06515	1346359..1346805	+	447	WP_028014956.1	GNAT family N-acetyltransferase	-
FBF68_RS06520	1346868..1347701	+	834	WP_028014955.1	DMT family transporter	-
FBF68_RS06525	1347877..1349061	+	1185	WP_039024559.1	multidrug efflux MFS transporter EmrD	-
FBF68_RS06530	1348964..1350052	-	1089	WP_063155235.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (27-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12447.64 Da
• Isoelectric Point: 10.2219

>T123973 WP_063155234.1 NZ_CP039452:c1345717-1345403 [Enterobacter bugandensis]
MHLISMKAIIDAVKLFPQHKEEFLFLGRTIEKIHCPTPAALKQIFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T123973 NZ_CP039452:c1345717-1345403 [Enterobacter bugandensis]
ATGCATTTAATTTCTATGAAGGCAATTATTGATGCGGTAAAACTGTTCCCGCAGCACAAGGAAGAGTTCCTTTTTTTAGG
TCGCACTATCGAAAAGATCCATTGCCCGACACCTGCTGCGCTGAAACAAATTTTTCCGACGCTGGATAACTTTAAATATC
TGGATAAGCATTATGTGATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTC
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15578.16 Da
• Isoelectric Point: 4.9534

>AT123973 WP_127334638.1 NZ_CP039452:c1345406-1344987 [Enterobacter bugandensis]
MMIVADAMKATHALVEAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARISRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT123973 NZ_CP039452:c1345406-1344987 [Enterobacter bugandensis]
ATGATGATCGTCGCTGATGCAATGAAAGCAACGCATGCCCTAGTCGAGGCTGTCCCTTTACTGGGTGAACATCCGAATGA
GAAAGATTACCAGGATGCACTTGAGTTGGTTGAGTATCTGCTCATGAATGAACCTGGTAGTCCTTTGCTGGATATCGTAT
GTGCAAGAATAAGTCGTTATGAAGCGAATCGACCCGAGATTGTCGCCTTGCGTCAGGAGATGGAATCTGTTCCAGTGGGA
ATTGCGGTTTTAAGAACCCTTATGGATCAGTACAAACTGACCATTTCAGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTATTGAATGGACAGCGTCAGCTGACGTTAAACCATATAAAAAAACTGGCGGTGCGCTTTGGGGTATCGC
CTGCACTGTTTATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A822X036