Detailed information of TA system TA123961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 900501..900915
• Replicon: chromosome
• Accession: NZ_CP039448
• Organism: Staphylococcus aureus strain VGC1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E8M03_RS04470
• Protein ID: WP_001065064.1
• Coordinates: 900501..900752 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E8M03_RS14680
• Protein ID: WP_000028424.1
• Coordinates: 900742..900915 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8M03_RS04425	895616..896290	+	675	WP_031769015.1	phi PV83 orf 19-like protein	-
E8M03_RS04430	896396..897253	-	858	WP_000371250.1	DUF4393 domain-containing protein	-
E8M03_RS04435	897318..898088	+	771	WP_031769013.1	conserved phage C-terminal domain-containing protein	-
E8M03_RS04440	898098..898871	+	774	WP_031769012.1	ATP-binding protein	-
E8M03_RS14675	898865..899026	+	162	WP_000237153.1	hypothetical protein	-
E8M03_RS04445	899039..899260	+	222	WP_001123673.1	DUF3269 family protein	-
E8M03_RS04450	899271..899675	+	405	WP_000049784.1	DUF1064 domain-containing protein	-
E8M03_RS04455	899680..899865	+	186	WP_001187242.1	DUF3113 family protein	-
E8M03_RS04460	899866..900237	+	372	WP_001140289.1	hypothetical protein	-
E8M03_RS04465	900238..900486	+	249	WP_001126846.1	phi PVL orf 51-like protein	-
E8M03_RS04470	900501..900752	+	252	WP_001065064.1	DUF1024 family protein	Toxin
E8M03_RS14680	900742..900915	+	174	WP_000028424.1	hypothetical protein	Antitoxin
E8M03_RS14685	900916..901077	+	162	WP_000889680.1	hypothetical protein	-
E8M03_RS04475	901092..901619	+	528	WP_149569683.1	dUTPase	-
E8M03_RS04480	901656..901862	+	207	WP_029625633.1	DUF1381 domain-containing protein	-
E8M03_RS04485	901859..902032	+	174	WP_149569684.1	transcriptional activator RinB	-
E8M03_RS04490	902033..902434	+	402	WP_000286968.1	hypothetical protein	-
E8M03_RS04500	902789..903283	+	495	WP_000594088.1	terminase small subunit	-
E8M03_RS04505	903276..904499	+	1224	WP_001037578.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selk / selq / seb	845250..934685	89435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9377.26 Da
• Isoelectric Point: 3.7128

>T123961 WP_001065064.1 NZ_CP039448:900501-900752 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T123961 NZ_CP039448:900501-900752 [Staphylococcus aureus]
ATGAATAATCGTGAACAAATTGAACAATCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGACAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT123961 WP_000028424.1 NZ_CP039448:900742-900915 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT123961 NZ_CP039448:900742-900915 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAATCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA