Detailed information of TA system TA123928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 4693872..4695190
• Replicon: chromosome
• Accession: NZ_CP039404
• Organism: Escherichia coli strain 377323_2f

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: E9097_RS23640
• Protein ID: WP_062860100.1
• Coordinates: 4693872..4694879 (-)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A2A7M399
• Locus tag: E9097_RS23645
• Protein ID: WP_001490601.1
• Coordinates: 4694879..4695190 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E9097_RS23605	4689565..4689756	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
E9097_RS23610	4689753..4691432	+	1680	WP_021560079.1	cellulose biosynthesis protein BcsG	-
E9097_RS23615	4691518..4691625	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
-	4691674..4691739	+	66	NuclAT_24	-	-
-	4691674..4691739	+	66	NuclAT_24	-	-
-	4691674..4691739	+	66	NuclAT_24	-	-
-	4691674..4691739	+	66	NuclAT_24	-	-
E9097_RS23625	4692001..4692108	-	108	WP_000170747.1	type I toxin-antitoxin system toxin Ldr family protein	-
-	4692157..4692222	+	66	NuclAT_25	-	-
-	4692157..4692222	+	66	NuclAT_25	-	-
-	4692157..4692222	+	66	NuclAT_25	-	-
-	4692157..4692222	+	66	NuclAT_25	-	-
E9097_RS23635	4692584..4693855	+	1272	WP_001312176.1	amino acid permease	-
E9097_RS23640	4693872..4694879	-	1008	WP_062860100.1	HipA domain-containing protein	Toxin
E9097_RS23645	4694879..4695190	-	312	WP_001490601.1	HipA N-terminal domain-containing protein	Antitoxin
E9097_RS23650	4695175..4695498	-	324	WP_021560081.1	helix-turn-helix transcriptional regulator	-
E9097_RS23655	4695723..4696736	-	1014	WP_001357946.1	dipeptide ABC transporter ATP-binding subunit DppF	-
E9097_RS23660	4696733..4697716	-	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	-
E9097_RS23665	4697727..4698629	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
E9097_RS23670	4698639..4699658	-	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-302)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 38453.69 Da
• Isoelectric Point: 5.2501

>T123928 WP_062860100.1 NZ_CP039404:c4694879-4693872 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDYKGLPVHQEQLDGAMQITD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPTLAPVYDFVSVAPY
PEYFYSNYLELPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Nucleotide

• Length: 1008 bp

>T123928 NZ_CP039404:c4694879-4693872 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGACCCCCAGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGCTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATTACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTACAGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGTTTAGTGTACTCCGATGGTTTGACGCCAACGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTTACTCAAACTATCTGGAGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAACTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11822.40 Da
• Isoelectric Point: 6.0609

>AT123928 WP_001490601.1 NZ_CP039404:c4695190-4694879 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAQDYHGPAISISLPVAQRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Nucleotide

• Length: 312 bp

>AT123928 NZ_CP039404:c4695190-4694879 [Escherichia coli]
ATGCATCGACGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTACGCCCAGGATTACCACGGACCGGCAATCTCAATCAGCCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGACGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCAATCCAGATTTTACCTTGGGAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2A7M399