Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2998966..2999468 Replicon chromosome
Accession NZ_CP039404
Organism Escherichia coli strain 377323_2f

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E9097_RS15425 Protein ID WP_000767829.1
Coordinates 2998966..2999220 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E9097_RS15430 Protein ID WP_001259255.1
Coordinates 2999217..2999468 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E9097_RS15405 2995459..2996817 - 1359 WP_053880142.1 putrescine/proton symporter PlaP -
E9097_RS15410 2996807..2996869 - 63 WP_010723108.1 membrane protein YoeI -
E9097_RS15415 2997084..2998013 - 930 WP_000803366.1 LysR family transcriptional regulator -
E9097_RS15420 2998059..2998883 - 825 WP_021524296.1 SDR family oxidoreductase -
E9097_RS15425 2998966..2999220 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E9097_RS15430 2999217..2999468 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E9097_RS15440 2999751..2999801 + 51 WP_001364200.1 his operon leader peptide -
E9097_RS15445 2999947..3000846 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E9097_RS15450 3000852..3002156 + 1305 WP_032344119.1 histidinol dehydrogenase -
E9097_RS15455 3002153..3003223 + 1071 WP_053894041.1 histidinol-phosphate transaminase -
E9097_RS15460 3003223..3004290 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123921 WP_000767829.1 NZ_CP039404:c2999220-2998966 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123921 NZ_CP039404:c2999220-2998966 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAAGGGAAACCAGAACCACTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123921 WP_001259255.1 NZ_CP039404:c2999468-2999217 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123921 NZ_CP039404:c2999468-2999217 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References