Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 210890..211492 Replicon chromosome
Accession NZ_CP039404
Organism Escherichia coli strain 377323_2f

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag E9097_RS01005 Protein ID WP_000897302.1
Coordinates 210890..211201 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E9097_RS01010 Protein ID WP_000356397.1
Coordinates 211202..211492 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E9097_RS00980 206804..207403 + 600 WP_001298594.1 glucose-1-phosphatase -
E9097_RS00985 207397..208269 + 873 WP_000920762.1 virulence factor BrkB family protein -
E9097_RS00990 208266..208703 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
E9097_RS00995 208748..209689 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
E9097_RS01000 209753..210661 - 909 WP_001305059.1 alpha/beta hydrolase -
E9097_RS01005 210890..211201 + 312 WP_000897302.1 hypothetical protein Toxin
E9097_RS01010 211202..211492 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
E9097_RS01015 212096..212314 + 219 WP_001314326.1 ribbon-helix-helix domain-containing protein -
E9097_RS01020 212538..213467 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
E9097_RS01025 213464..214099 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
E9097_RS01030 214096..214998 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T123903 WP_000897302.1 NZ_CP039404:210890-211201 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T123903 NZ_CP039404:210890-211201 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT123903 WP_000356397.1 NZ_CP039404:211202-211492 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT123903 NZ_CP039404:211202-211492 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References