Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2868316..2868818 Replicon chromosome
Accession NZ_CP039403
Organism Escherichia coli strain 382634_2f

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E4311_RS14480 Protein ID WP_000767829.1
Coordinates 2868316..2868570 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E4311_RS14485 Protein ID WP_001259255.1
Coordinates 2868567..2868818 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4311_RS14460 2864809..2866167 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E4311_RS14465 2866157..2866219 - 63 WP_010723108.1 membrane protein YoeI -
E4311_RS14470 2866434..2867363 - 930 WP_000803366.1 LysR family transcriptional regulator -
E4311_RS14475 2867409..2868233 - 825 WP_021524296.1 SDR family oxidoreductase -
E4311_RS14480 2868316..2868570 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E4311_RS14485 2868567..2868818 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E4311_RS14495 2869101..2869151 + 51 WP_001364200.1 his operon leader peptide -
E4311_RS14500 2869297..2870196 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E4311_RS14505 2870202..2871506 + 1305 WP_032344119.1 histidinol dehydrogenase -
E4311_RS14510 2871503..2872573 + 1071 WP_097560999.1 histidinol-phosphate transaminase -
E4311_RS14515 2872573..2873640 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123895 WP_000767829.1 NZ_CP039403:c2868570-2868316 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123895 NZ_CP039403:c2868570-2868316 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAAGGGAAACCAGAACCACTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123895 WP_001259255.1 NZ_CP039403:c2868818-2868567 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123895 NZ_CP039403:c2868818-2868567 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References