Detailed information of TA system TA123878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 212309..212911
• Replicon: chromosome
• Accession: NZ_CP039403
• Organism: Escherichia coli strain 382634_2f

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: E4311_RS01010
• Protein ID: WP_000897302.1
• Coordinates: 212309..212620 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4311_RS01015
• Protein ID: WP_000356397.1
• Coordinates: 212621..212911 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4311_RS00985	208223..208822	+	600	WP_001298594.1	glucose-1-phosphatase	-
E4311_RS00990	208816..209688	+	873	WP_000920762.1	virulence factor BrkB family protein	-
E4311_RS00995	209685..210122	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
E4311_RS01000	210167..211108	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
E4311_RS01005	211172..212080	-	909	WP_001305059.1	alpha/beta hydrolase	-
E4311_RS01010	212309..212620	+	312	WP_000897302.1	hypothetical protein	Toxin
E4311_RS01015	212621..212911	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
E4311_RS01020	213515..213733	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
E4311_RS01025	213957..214886	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
E4311_RS01030	214883..215518	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
E4311_RS01035	215515..216417	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T123878 WP_000897302.1 NZ_CP039403:212309-212620 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T123878 NZ_CP039403:212309-212620 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT123878 WP_000356397.1 NZ_CP039403:212621-212911 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT123878 NZ_CP039403:212621-212911 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416