Detailed information of TA system TA123859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1673448..1674178
• Replicon: chromosome
• Accession: NZ_CP039388
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: EZJ11_RS08095
• Protein ID: WP_023302825.1
• Coordinates: 1673864..1674178 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EZJ11_RS08090
• Protein ID: WP_032620773.1
• Coordinates: 1673448..1673864 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS08065 (EZJ11_08210)	1668897..1670402	-	1506	WP_032634219.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
EZJ11_RS08070 (EZJ11_08215)	1670399..1670992	-	594	WP_003861054.1	transcriptional regulator UhpA	-
EZJ11_RS08075 (EZJ11_08220)	1671118..1671405	-	288	WP_015570113.1	acetolactate synthase small subunit	-
EZJ11_RS08080 (EZJ11_08225)	1671409..1673096	-	1688	Protein_1573	acetolactate synthase large subunit	-
EZJ11_RS08085 (EZJ11_08230)	1673203..1673301	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
EZJ11_RS08090 (EZJ11_08235)	1673448..1673864	-	417	WP_032620773.1	helix-turn-helix domain-containing protein	Antitoxin
EZJ11_RS08095 (EZJ11_08240)	1673864..1674178	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
EZJ11_RS08100 (EZJ11_08245)	1674680..1674754	+	75	WP_223237555.1	type I toxin-antitoxin system toxin TisB	-
EZJ11_RS08105 (EZJ11_08250)	1674819..1675265	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
EZJ11_RS08110 (EZJ11_08255)	1675328..1676161	+	834	WP_032620535.1	DMT family transporter	-
EZJ11_RS08115 (EZJ11_08260)	1676337..1677521	+	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
EZJ11_RS08120 (EZJ11_08265)	1677427..1678512	-	1086	Protein_1581	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T123859 WP_023302825.1 NZ_CP039388:c1674178-1673864 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T123859 NZ_CP039388:c1674178-1673864 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15249.77 Da
• Isoelectric Point: 5.2068

>AT123859 WP_032620773.1 NZ_CP039388:c1673864-1673448 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT123859 NZ_CP039388:c1673864-1673448 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGTGCG
CCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2