Detailed information of TA system TA123857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 439583..440258
• Replicon: chromosome
• Accession: NZ_CP039388
• Organism: Enterobacter hormaechei strain cre64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EZJ11_RS02020
• Protein ID: WP_032619177.1
• Coordinates: 439583..439882 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: EZJ11_RS02025
• Protein ID: WP_015571639.1
• Coordinates: 439893..440258 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZJ11_RS02000 (EZJ11_02030)	434717..435334	-	618	WP_032634496.1	DUF1819 family protein	-
EZJ11_RS02005 (EZJ11_02035)	435498..436466	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
EZJ11_RS02010 (EZJ11_02040)	436499..438154	+	1656	Protein_388	ATP-binding cassette domain-containing protein	-
EZJ11_RS02015 (EZJ11_02045)	438135..439332	+	1198	Protein_389	HlyD family efflux transporter periplasmic adaptor subunit	-
EZJ11_RS02020 (EZJ11_02050)	439583..439882	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZJ11_RS02025 (EZJ11_02055)	439893..440258	+	366	WP_015571639.1	HigA family addiction module antitoxin	Antitoxin
EZJ11_RS02030 (EZJ11_02060)	440285..441465	-	1181	Protein_392	PLP-dependent aminotransferase family protein	-
EZJ11_RS02035 (EZJ11_02065)	441485..442372	-	888	WP_032619173.1	LysR family transcriptional regulator	-
EZJ11_RS02040 (EZJ11_02070)	442470..443072	+	603	WP_017382759.1	short chain dehydrogenase	-
EZJ11_RS02045 (EZJ11_02075)	443069..443800	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	435498..461210	25712
	-	flank	IS/Tn	-	-	435498..436421	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T123857 WP_032619177.1 NZ_CP039388:439583-439882 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T123857 NZ_CP039388:439583-439882 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT123857 WP_015571639.1 NZ_CP039388:439893-440258 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT123857 NZ_CP039388:439893-440258 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9