Detailed information of TA system TA123846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1132899..1133467
• Replicon: chromosome
• Accession: NZ_CP039374
• Organism: Halomonas titanicae strain ANRCS81

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1G8P1L0
• Locus tag: E8A47_RS05050
• Protein ID: WP_022522161.1
• Coordinates: 1132899..1133177 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E8A47_RS05055
• Protein ID: WP_022522160.1
• Coordinates: 1133186..1133467 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8A47_RS05035	1128557..1129849	-	1293	WP_041157725.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
E8A47_RS05040	1129873..1130796	-	924	WP_141441456.1	acetyltransferase	-
E8A47_RS05045	1131151..1132863	+	1713	WP_208797180.1	cyclic peptide export ABC transporter	-
E8A47_RS05050	1132899..1133177	+	279	WP_022522161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E8A47_RS05055	1133186..1133467	+	282	WP_022522160.1	HigA family addiction module antidote protein	Antitoxin
E8A47_RS05060	1133523..1133729	-	207	Protein_991	hypothetical protein	-
E8A47_RS05065	1133803..1135002	-	1200	WP_141439797.1	hypothetical protein	-
E8A47_RS05070	1134999..1138391	-	3393	WP_141439796.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1127546..1140443	12897

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10504.94 Da
• Isoelectric Point: 10.0038

>T123846 WP_022522161.1 NZ_CP039374:1132899-1133177 [Halomonas titanicae]
MIKNFRHKGLRKFFETGSTSGIQAKHATKLQIQLTALDSAKGPEDMNAPGWRLHPLSHKLKGHWSITVNGNWRLTFAFEG
EDAILVDYQDYH

Nucleotide

• Length: 279 bp

>T123846 NZ_CP039374:1132899-1133177 [Halomonas titanicae]
ATGATCAAGAACTTTCGACACAAAGGCCTGCGCAAGTTCTTCGAAACCGGCTCAACTTCCGGTATTCAGGCCAAGCACGC
GACCAAGTTGCAAATCCAGCTAACCGCGCTGGACAGTGCAAAGGGCCCTGAGGATATGAATGCACCGGGTTGGAGGCTGC
ATCCATTGTCTCACAAGCTGAAAGGACACTGGTCTATCACTGTTAACGGGAACTGGCGGCTCACCTTCGCTTTCGAAGGC
GAGGATGCCATCCTCGTTGATTATCAAGACTATCACTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10210.68 Da
• Isoelectric Point: 8.6800

>AT123846 WP_022522160.1 NZ_CP039374:1133186-1133467 [Halomonas titanicae]
MNRMHNPAHPGTVLREYIGDISVTQAAQQLGVTRTALSRILNGNAGISADMALRLEQALGTSAEMWLEMQLKYELWQASQ
RPRAQVTPLHAAG

Nucleotide

• Length: 282 bp

>AT123846 NZ_CP039374:1133186-1133467 [Halomonas titanicae]
ATGAATCGTATGCATAATCCCGCGCATCCTGGCACTGTTTTGCGCGAGTACATTGGCGATATCTCTGTTACCCAGGCGGC
CCAGCAGCTTGGCGTGACGCGTACTGCGCTATCGCGGATTCTGAACGGTAATGCCGGTATTTCTGCCGACATGGCACTGA
GGCTTGAGCAAGCATTGGGTACCAGCGCCGAAATGTGGCTGGAAATGCAGCTCAAGTATGAGCTATGGCAAGCGTCACAG
CGTCCACGCGCTCAGGTCACCCCTCTTCACGCGGCGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A1G8P1L0