Detailed information of TA system TA123833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1942813..1943428
• Replicon: chromosome
• Accession: NZ_CP039371
• Organism: Pseudomonas putida strain 1290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E6B08_RS09220
• Protein ID: WP_136913723.1
• Coordinates: 1942813..1943109 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6B08_RS09225
• Protein ID: WP_136913724.1
• Coordinates: 1943111..1943428 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6B08_RS09190	1938153..1938653	+	501	WP_136913718.1	hypothetical protein	-
E6B08_RS09195	1938665..1938907	+	243	WP_136913719.1	hypothetical protein	-
E6B08_RS09200	1939162..1939425	-	264	WP_136913720.1	DUF3077 domain-containing protein	-
E6B08_RS09205	1940148..1940297	-	150	WP_136913721.1	CopG family transcriptional regulator	-
E6B08_RS09210	1940389..1941588	-	1200	WP_136913722.1	MFS transporter	-
E6B08_RS09215	1941710..1941973	-	264	WP_136917360.1	DUF3077 domain-containing protein	-
E6B08_RS09220	1942813..1943109	+	297	WP_136913723.1	DNA-binding protein	Toxin
E6B08_RS09225	1943111..1943428	+	318	WP_136913724.1	helix-turn-helix domain-containing protein	Antitoxin
E6B08_RS09230	1944103..1944633	+	531	WP_136913725.1	VOC family protein	-
E6B08_RS09235	1944819..1945496	-	678	WP_136913726.1	GntR family transcriptional regulator	-
E6B08_RS09240	1945734..1946552	+	819	WP_136913727.1	enoyl-CoA hydratase/isomerase family protein	-
E6B08_RS09245	1946554..1947750	+	1197	WP_202877488.1	CoA transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10867.40 Da
• Isoelectric Point: 8.5311

>T123833 WP_136913723.1 NZ_CP039371:1942813-1943109 [Pseudomonas putida]
MRTVIETPTFQKNAAKLWTDEERVAFIDWISANPSAGDVIQGADGARKVRWRRAGSGKSGGVRVIYFNLTEDEVVLLIMV
YAKAERSNVDPAEISKVV

Nucleotide

• Length: 297 bp

>T123833 NZ_CP039371:1942813-1943109 [Pseudomonas putida]
ATGCGAACAGTGATCGAAACACCGACATTCCAGAAAAACGCTGCCAAACTCTGGACTGATGAGGAACGTGTAGCGTTCAT
CGATTGGATTTCGGCCAACCCGAGCGCTGGGGACGTCATCCAAGGCGCGGATGGTGCGCGAAAGGTGCGCTGGCGTCGTG
CTGGTTCGGGTAAATCAGGCGGAGTAAGGGTGATTTATTTCAATCTCACCGAAGATGAAGTCGTGTTACTGATCATGGTC
TATGCAAAGGCAGAACGATCCAATGTAGACCCAGCTGAAATCAGCAAGGTGGTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11474.21 Da
• Isoelectric Point: 6.9842

>AT123833 WP_136913724.1 NZ_CP039371:1943111-1943428 [Pseudomonas putida]
MRDIDIEAVAKAIEADAGEALPDLRQALKEAKAGAGRVSTPEQILVRQARERSGLTQASFAKRIDTPVATLRDWEQGRFV
PPGGVLCLLRLIVKHPELSQELATF

Nucleotide

• Length: 318 bp

>AT123833 NZ_CP039371:1943111-1943428 [Pseudomonas putida]
ATGCGTGACATCGATATCGAAGCCGTCGCCAAGGCCATTGAGGCAGATGCAGGCGAGGCGTTGCCTGATCTACGCCAGGC
TCTAAAAGAAGCGAAAGCGGGTGCTGGTCGGGTCAGTACGCCGGAGCAGATACTGGTACGGCAGGCGCGTGAGCGTTCCG
GGTTGACTCAGGCTTCCTTTGCCAAGCGTATCGACACGCCCGTCGCAACCCTTAGAGATTGGGAACAGGGGCGTTTCGTG
CCGCCTGGTGGTGTACTTTGCCTGTTGCGTCTGATCGTCAAGCATCCTGAGCTTTCGCAGGAACTGGCTACTTTCTGA