Detailed information of TA system TA123826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 196311..197152
• Replicon: plasmid pUW386
• Accession: NZ_CP039340
• Organism: Ralstonia solanacearum strain UW386

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: E7Z57_RS17965
• Protein ID: WP_138929827.1
• Coordinates: 196637..197152 (+)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0S4TWF3
• Locus tag: E7Z57_RS17960
• Protein ID: WP_064051187.1
• Coordinates: 196311..196640 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z57_RS17945	192312..193898	-	1587	WP_064051190.1	aldehyde dehydrogenase (NADP(+))	-
E7Z57_RS17950	193930..194865	-	936	WP_138929825.1	5-dehydro-4-deoxyglucarate dehydratase	-
E7Z57_RS17955	195169..196029	+	861	WP_138929826.1	spermidine synthase	-
E7Z57_RS17960	196311..196640	+	330	WP_064051187.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
E7Z57_RS17965	196637..197152	+	516	WP_138929827.1	type II toxin-antitoxin system YhaV family toxin	Toxin
E7Z57_RS17975	197411..198307	+	897	WP_138929828.1	LysR family transcriptional regulator	-
E7Z57_RS17980	198553..199431	+	879	WP_138929829.1	EamA family transporter	-
E7Z57_RS17985	199947..201176	+	1230	WP_064051182.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / fliI / fliM / flgG / flgC / cyaB / motA / cheW / cheB / cheY / adeG	1..1830097	1830097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 19774.57 Da
• Isoelectric Point: 10.0946

>T123826 WP_138929827.1 NZ_CP039340:196637-197152 [Ralstonia solanacearum]
MTAIRPVPLVIHGWTIFAHPLFLDQLDALTRQVEALREKDPGGYVKKNATKRLTAICKLAFDVIPQDPARPEYRQGHTLG
DENKHWFRTKFFQQYRLFFRFHAPSKMIVLAWINNADTRRAYESDDDAYRVFKRMLASGHPPNDWEQLLTEAGRQSERLQ
SVAGALGTGHR

Nucleotide

• Length: 516 bp

>T123826 NZ_CP039340:196637-197152 [Ralstonia solanacearum]
ATGACGGCTATCCGACCGGTTCCTTTGGTTATCCACGGCTGGACGATCTTCGCGCATCCGCTGTTCCTCGATCAGCTTGA
CGCCCTGACCCGGCAGGTGGAGGCCCTGCGAGAAAAAGACCCCGGTGGGTACGTCAAGAAGAACGCCACCAAGCGGCTGA
CCGCCATCTGCAAGTTGGCGTTCGATGTCATCCCGCAAGACCCCGCACGACCTGAATACCGCCAAGGTCATACCCTTGGC
GACGAGAACAAGCATTGGTTTCGCACCAAGTTCTTCCAGCAGTATCGGTTGTTCTTCCGGTTCCATGCGCCCAGCAAGAT
GATCGTGCTGGCCTGGATCAACAACGCGGACACGAGGCGAGCCTACGAGAGCGATGACGATGCTTACCGCGTCTTCAAGC
GAATGCTGGCCAGCGGCCACCCGCCGAACGACTGGGAGCAGCTGCTGACAGAGGCGGGGCGCCAATCCGAGCGCTTGCAG
AGCGTGGCCGGCGCGCTGGGTACTGGCCATCGTTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12068.51 Da
• Isoelectric Point: 4.4731

>AT123826 WP_064051187.1 NZ_CP039340:196311-196640 [Ralstonia solanacearum]
MPATLEAESTLTDRYQTTVPETVRRALRLGKRDKIHYTIRQTGEVVLTRADDDEAGDPVLGQFLRFLAQDIAAHPERLQG
LDTSLVQRIHTLVGGIEVDLDAALSADDE

Nucleotide

• Length: 330 bp

>AT123826 NZ_CP039340:196311-196640 [Ralstonia solanacearum]
ATGCCCGCGACGCTTGAAGCCGAGTCCACGCTGACCGACCGCTACCAGACCACGGTTCCAGAAACCGTCCGCCGCGCCTT
GCGCTTGGGCAAGCGCGACAAGATTCACTACACCATCCGCCAAACCGGCGAAGTGGTGCTGACCCGCGCCGATGACGATG
AAGCCGGCGATCCGGTGCTTGGCCAATTCCTTCGCTTCCTGGCCCAGGACATCGCCGCACATCCTGAACGTCTGCAAGGC
TTGGATACCAGCCTGGTGCAACGCATCCACACGCTGGTTGGCGGCATCGAGGTGGACCTGGATGCCGCACTGTCGGCGGA
CGATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4TWF3