Detailed information of TA system TA123819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 51978..52705
• Replicon: plasmid unnamed2
• Accession: NZ_CP039329
• Organism: Citrobacter portucalensis strain Effluent_1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: E7703_RS25600
• Protein ID: WP_000558568.1
• Coordinates: 51978..52289 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: E7703_RS25605
• Protein ID: WP_000990392.1
• Coordinates: 52286..52705 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7703_RS25580	48492..49211	+	720	WP_001102107.1	(Fe-S)-binding protein	-
E7703_RS25585	49222..50649	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
E7703_RS25590	50642..51337	+	696	WP_000370412.1	lactate utilization protein C	-
E7703_RS25595	51387..51767	-	381	Protein_56	gluconate permease	-
E7703_RS25600	51978..52289	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
E7703_RS25605	52286..52705	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
E7703_RS25610	52742..52951	-	210	WP_020806042.1	hypothetical protein	-
E7703_RS25620	53060..53764	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
E7703_RS25625	53829..54833	+	1005	Protein_61	conjugative transfer relaxase/helicase TraI	-
E7703_RS25630	54853..55599	+	747	WP_021553161.1	conjugal transfer pilus acetylase TraX	-
E7703_RS25635	55654..56214	+	561	WP_016240418.1	fertility inhibition protein FinO	-
E7703_RS25640	56347..56559	+	213	WP_008786559.1	hypothetical protein	-
E7703_RS25645	56732..56968	-	237	WP_113990385.1	hypothetical protein	-
E7703_RS25650	56920..57129	+	210	WP_001299729.1	hemolysin expression modulator Hha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..66363	66363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T123819 WP_000558568.1 NZ_CP039329:51978-52289 [Citrobacter portucalensis]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T123819 NZ_CP039329:51978-52289 [Citrobacter portucalensis]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT123819 WP_000990392.1 NZ_CP039329:52286-52705 [Citrobacter portucalensis]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT123819 NZ_CP039329:52286-52705 [Citrobacter portucalensis]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4