Detailed information of TA system TA123803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2446895..2447457
• Replicon: chromosome
• Accession: NZ_CP039327
• Organism: Citrobacter portucalensis strain Effluent_1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E7703_RS12075
• Protein ID: WP_048216313.1
• Coordinates: 2446895..2447173 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: E7703_RS12080
• Protein ID: WP_003833018.1
• Coordinates: 2447173..2447457 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7703_RS12055	2441972..2442403	-	432	WP_003833007.1	peroxiredoxin OsmC	-
E7703_RS12060	2442631..2442771	+	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
E7703_RS12065	2442951..2444648	+	1698	WP_003833011.1	malate dehydrogenase	-
E7703_RS12070	2444958..2446673	+	1716	WP_136391886.1	ATP-binding cassette domain-containing protein	-
E7703_RS12075	2446895..2447173	+	279	WP_048216313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E7703_RS12080	2447173..2447457	+	285	WP_003833018.1	HigA family addiction module antidote protein	Antitoxin
E7703_RS12085	2447497..2448099	-	603	WP_003833020.1	inorganic diphosphatase	-
E7703_RS12090	2448229..2448885	-	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
E7703_RS12095	2448878..2449762	-	885	WP_003833024.1	formate dehydrogenase N subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10681.33 Da
• Isoelectric Point: 9.8965

>T123803 WP_048216313.1 NZ_CP039327:2446895-2447173 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTEINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T123803 NZ_CP039327:2446895-2447173 [Citrobacter portucalensis]
ATGATCATGAACTTCAGGCACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGACTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGAGATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTGGGTGAACGCGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGATTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT123803 WP_003833018.1 NZ_CP039327:2447173-2447457 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT123803 NZ_CP039327:2447173-2447457 [Citrobacter portucalensis]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCGTCAACGGCAAGTCGACTATTAACCGGGAAAGCGGCTCTGACGCCTGAGATGGCGA
TTAAACTCTCCGTAGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGTCTGCGTAAGCTGGCGGTACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8