Detailed information of TA system TA123791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1958598..1959292
• Replicon: chromosome
• Accession: NZ_CP039298
• Organism: Escherichia coli strain PigCaeca_1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: E7Z58_RS09825
• Protein ID: WP_001263493.1
• Coordinates: 1958598..1958996 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E7Z58_RS09830
• Protein ID: WP_000554757.1
• Coordinates: 1958999..1959292 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E7Z58_RS09795	1953598..1954842	-	1245	WP_000189541.1	esterase FrsA	-
-	1954258..1954338	-	81	NuclAT_11	-	-
-	1954258..1954338	-	81	NuclAT_11	-	-
-	1954258..1954338	-	81	NuclAT_11	-	-
-	1954258..1954338	-	81	NuclAT_11	-	-
E7Z58_RS09800	1954934..1955392	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E7Z58_RS09805	1955653..1957110	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
E7Z58_RS09810	1957167..1957688	-	522	Protein_1890	peptide chain release factor H	-
E7Z58_RS09815	1957687..1957890	-	204	Protein_1891	RNA ligase RtcB family protein	-
E7Z58_RS09820	1958136..1958588	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
E7Z58_RS09825	1958598..1958996	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E7Z58_RS09830	1958999..1959292	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E7Z58_RS09835	1959344..1960399	-	1056	WP_001226164.1	DNA polymerase IV	-
E7Z58_RS09840	1960470..1961255	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
E7Z58_RS09845	1961227..1962939	+	1713	Protein_1897	flagellar biosynthesis protein FlhA	-
E7Z58_RS09850	1963163..1963660	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	1910401..1980655	70254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T123791 WP_001263493.1 NZ_CP039298:c1958996-1958598 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T123791 NZ_CP039298:c1958996-1958598 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT123791 WP_000554757.1 NZ_CP039298:c1959292-1958999 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT123791 NZ_CP039298:c1959292-1958999 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6