Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 107478..107980 Replicon chromosome
Accession NZ_CP039298
Organism Escherichia coli strain PigCaeca_1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E7Z58_RS00530 Protein ID WP_000767829.1
Coordinates 107726..107980 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E7Z58_RS00525 Protein ID WP_001259255.1
Coordinates 107478..107729 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E7Z58_RS00500 102656..103723 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E7Z58_RS00505 103723..104793 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
E7Z58_RS00510 104790..106094 - 1305 WP_001593467.1 histidinol dehydrogenase -
E7Z58_RS00515 106100..106999 - 900 WP_094304651.1 ATP phosphoribosyltransferase -
E7Z58_RS00520 107145..107195 - 51 WP_001364200.1 his operon leader peptide -
E7Z58_RS00525 107478..107729 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E7Z58_RS00530 107726..107980 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E7Z58_RS00535 108063..108887 + 825 WP_000754758.1 SDR family oxidoreductase -
E7Z58_RS00540 108933..109862 + 930 WP_000803370.1 LysR family transcriptional regulator -
E7Z58_RS00545 110077..110139 + 63 WP_010723108.1 membrane protein YoeI -
E7Z58_RS00550 110129..111487 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E7Z58_RS00555 111666..112724 + 1059 WP_000492346.1 transport protein YeeE -
E7Z58_RS00560 112738..112965 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123779 WP_000767829.1 NZ_CP039298:107726-107980 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123779 NZ_CP039298:107726-107980 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123779 WP_001259255.1 NZ_CP039298:107478-107729 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123779 NZ_CP039298:107478-107729 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References