Detailed information of TA system TA123746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5822113..5822708
• Replicon: chromosome
• Accession: NZ_CP039293
• Organism: Pseudomonas aeruginosa strain PABL048

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: DZ927_RS28085
• Protein ID: WP_003113526.1
• Coordinates: 5822430..5822708 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DZ927_RS28080
• Protein ID: WP_003133769.1
• Coordinates: 5822113..5822418 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DZ927_RS28045	5817254..5818102	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
DZ927_RS28055	5818269..5819210	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
DZ927_RS28060	5819327..5819941	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
DZ927_RS28065	5819983..5820567	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
DZ927_RS28070	5820608..5821708	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
DZ927_RS28080	5822113..5822418	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
DZ927_RS28085	5822430..5822708	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DZ927_RS28095	5823037..5825265	+	2229	WP_019485341.1	TonB-dependent receptor	-
DZ927_RS28100	5825335..5825982	-	648	WP_003095021.1	carbonate dehydratase	-
DZ927_RS28105	5826044..5827282	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T123746 WP_003113526.1 NZ_CP039293:c5822708-5822430 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T123746 NZ_CP039293:c5822708-5822430 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT123746 WP_003133769.1 NZ_CP039293:c5822418-5822113 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT123746 NZ_CP039293:c5822418-5822113 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3