Detailed information of TA system TA123728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1775891..1776516
• Replicon: chromosome
• Accession: NZ_CP039288
• Organism: Cupriavidus necator H16

Toxin (Protein)

• Gene name: graT
• Uniprot ID: R7XAD8
• Locus tag: E6A55_RS27150
• Protein ID: WP_010814558.1
• Coordinates: 1775891..1776169 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6A55_RS27155
• Protein ID: WP_010814559.1
• Coordinates: 1776181..1776516 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6A55_RS27150	1775891..1776169	+	279	WP_010814558.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6A55_RS27155	1776181..1776516	+	336	WP_010814559.1	HigA family addiction module antidote protein	Antitoxin
E6A55_RS27160	1776568..1777938	-	1371	WP_011617316.1	serine hydrolase	-
E6A55_RS27165	1777985..1778464	-	480	WP_010814561.1	MarR family transcriptional regulator	-
E6A55_RS27170	1778643..1779239	-	597	WP_010814562.1	GTP cyclohydrolase II	-
E6A55_RS27175	1779508..1780038	+	531	WP_010814563.1	hypothetical protein	-
E6A55_RS27180	1780047..1780517	-	471	WP_010814564.1	Lrp/AsnC family transcriptional regulator	-
E6A55_RS27185	1780669..1781010	+	342	WP_010814565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10732.42 Da
• Isoelectric Point: 10.4151

>T123728 WP_010814558.1 NZ_CP039288:1775891-1776169 [Cupriavidus necator H16]
MIQSFRCSDTAELFAGRCVPRFGNIRAVAERKLRLLQRASTLEFLRSPPGNRLQLLTGDRKGRYSIRINEQWRICFIWTH
DGPAVVEIIDYH

Nucleotide

• Length: 279 bp

>T123728 NZ_CP039288:1775891-1776169 [Cupriavidus necator H16]
ATGATTCAGTCATTCAGATGTTCCGACACCGCTGAACTGTTCGCGGGCCGCTGTGTACCGCGCTTCGGCAATATCCGCGC
CGTGGCCGAACGCAAGCTGCGGCTGCTGCAGAGAGCGTCGACGCTGGAGTTCCTGCGCTCCCCGCCGGGCAATCGCCTGC
AGTTGCTGACCGGCGACCGCAAAGGCCGCTACAGCATCCGCATCAACGAGCAATGGCGAATCTGCTTCATCTGGACCCAC
GACGGCCCCGCGGTCGTTGAGATCATCGACTACCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12308.11 Da
• Isoelectric Point: 7.2042

>AT123728 WP_010814559.1 NZ_CP039288:1776181-1776516 [Cupriavidus necator H16]
MVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYD
LKVAQRDHGERIMAEVAPRDAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT123728 NZ_CP039288:1776181-1776516 [Cupriavidus necator H16]
ATGGTTCGCTTCAAGGACGGCATGCGTCCCATCCACCCGGGCGAAATCCTGCGCGAGGATTTCCTGGTACCGCTCAACAT
GAGCGCCAACGCCCTGGCCCTCGCGCTGCGCGTGACCGCGGCGCGCATCAACGACATCGTCCGCGAGCAACGTGGCATCA
CGCCGGACACCGCCCTGCGCCTGGCGCGCTATTTTGGCGGCGATGCGCAGTCGTGGCTGAACCTGCAGCTGGACTATGAC
CTGAAGGTCGCGCAGCGCGACCACGGCGAGCGCATCATGGCGGAAGTGGCGCCGCGCGATGCCGCGGCGGCCGGTCAACC
GGCCGCTGAAAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7T4G2P6