Detailed information of TA system TA123698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 44723..45271
• Replicon: plasmid unnamed3
• Accession: NZ_CP039243
• Organism: Piscirickettsia salmonis strain MR5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: PsalMR5_RS23500
• Protein ID: WP_155081858.1
• Coordinates: 44723..45007 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: PsalMR5_RS23505
• Protein ID: WP_032126795.1
• Coordinates: 45011..45271 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalMR5_RS23470	40305..41191	+	887	WP_155079826.1	IS630 family transposase	-
PsalMR5_RS23475	41566..41790	-	225	Protein_58	DDE-type integrase/transposase/recombinase	-
PsalMR5_RS23480	41875..42849	+	975	WP_155079445.1	IS30 family transposase	-
PsalMR5_RS23485	42886..43401	-	516	Protein_60	IS6 family transposase	-
PsalMR5_RS23490	43559..44035	+	477	WP_196214949.1	transposase	-
PsalMR5_RS23495	44208..44582	+	375	WP_155081857.1	Mov34/MPN/PAD-1 family protein	-
PsalMR5_RS23500	44723..45007	-	285	WP_155081858.1	Txe/YoeB family addiction module toxin	Toxin
PsalMR5_RS23505	45011..45271	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PsalMR5_RS23510	45543..45917	+	375	Protein_65	site-specific integrase	-
PsalMR5_RS23515	45974..46198	+	225	WP_155081859.1	transposase	-
PsalMR5_RS23520	46380..46688	+	309	WP_155079958.1	hypothetical protein	-
PsalMR5_RS23525	46685..47034	+	350	Protein_68	IS66 family insertion sequence element accessory protein TnpB	-
PsalMR5_RS23530	47077..48333	+	1257	WP_155081861.1	IS66 family transposase	-
PsalMR5_RS23535	48375..48557	+	183	WP_155081862.1	transposase domain-containing protein	-
PsalMR5_RS23540	48554..49045	+	492	WP_155081863.1	transposase	-
PsalMR5_RS23545	49201..49749	-	549	WP_155081864.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65228	65228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11193.79 Da
• Isoelectric Point: 8.9660

>T123698 WP_155081858.1 NZ_CP039243:c45007-44723 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHNFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQSLRV

Nucleotide

• Length: 285 bp

>T123698 NZ_CP039243:c45007-44723 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGCCAACGCACGCCGACAGAAGGTATTGGCCATCCCGAACCTTTAAAAC
ATAATTTCAGTGGTTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATCGCTAAGGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123698 WP_032126795.1 NZ_CP039243:c45271-45011 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123698 NZ_CP039243:c45271-45011 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCGCGGGATAATTTTAAGAGCACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B8U9U2