Detailed information of TA system TA123695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 43144..43692
• Replicon: plasmid unnamed4
• Accession: NZ_CP039238
• Organism: Piscirickettsia salmonis strain BI1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: PsalBI1_RS23785
• Protein ID: WP_155081858.1
• Coordinates: 43144..43428 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: PsalBI1_RS23790
• Protein ID: WP_032126795.1
• Coordinates: 43432..43692 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalBI1_RS23750	38189..38704	+	516	WP_155085418.1	transposase	-
PsalBI1_RS23755	38726..39612	+	887	WP_155079826.1	IS630 family transposase	-
PsalBI1_RS23760	39987..40211	-	225	Protein_62	DDE-type integrase/transposase/recombinase	-
PsalBI1_RS23765	40296..41270	+	975	WP_155079445.1	IS30 family transposase	-
PsalBI1_RS23770	41307..41822	-	516	Protein_64	IS6 family transposase	-
PsalBI1_RS23775	41980..42456	+	477	WP_196214949.1	transposase	-
PsalBI1_RS23780	42629..43003	+	375	WP_155081857.1	Mov34/MPN/PAD-1 family protein	-
PsalBI1_RS23785	43144..43428	-	285	WP_155081858.1	Txe/YoeB family addiction module toxin	Toxin
PsalBI1_RS23790	43432..43692	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PsalBI1_RS23795	43964..44338	+	375	Protein_69	site-specific integrase	-
PsalBI1_RS23800	44395..44619	+	225	WP_155081859.1	transposase	-
PsalBI1_RS23805	45089..45397	+	309	WP_155079958.1	hypothetical protein	-
PsalBI1_RS23810	45394..45747	+	354	WP_155085419.1	IS66 family insertion sequence element accessory protein TnpB	-
PsalBI1_RS23815	45786..47267	+	1482	WP_155080463.1	IS66 family transposase	-
PsalBI1_RS23820	47264..47755	+	492	WP_155081863.1	transposase	-
PsalBI1_RS23825	47911..48459	-	549	WP_155081864.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61348	61348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11193.79 Da
• Isoelectric Point: 8.9660

>T123695 WP_155081858.1 NZ_CP039238:c43428-43144 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHNFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQSLRV

Nucleotide

• Length: 285 bp

>T123695 NZ_CP039238:c43428-43144 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGCCAACGCACGCCGACAGAAGGTATTGGCCATCCCGAACCTTTAAAAC
ATAATTTCAGTGGTTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATCGCTAAGGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123695 WP_032126795.1 NZ_CP039238:c43692-43432 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123695 NZ_CP039238:c43692-43432 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCGCGGGATAATTTTAAGAGCACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B8U9U2