Detailed information of TA system TA123690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 45678..46226
• Replicon: plasmid unnamed3
• Accession: NZ_CP039230
• Organism: Piscirickettsia salmonis strain SR1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: PsalSR1_RS23675
• Protein ID: WP_155081858.1
• Coordinates: 45678..45962 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: PsalSR1_RS23680
• Protein ID: WP_032126795.1
• Coordinates: 45966..46226 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalSR1_RS23645	41260..42146	+	887	WP_155079826.1	IS630 family transposase	-
PsalSR1_RS23650	42521..42745	-	225	Protein_62	DDE-type integrase/transposase/recombinase	-
PsalSR1_RS23655	42830..43804	+	975	WP_155079445.1	IS30 family transposase	-
PsalSR1_RS23660	43841..44356	-	516	Protein_64	IS6 family transposase	-
PsalSR1_RS23665	44514..44990	+	477	WP_196214949.1	transposase	-
PsalSR1_RS23670	45163..45537	+	375	WP_155081857.1	Mov34/MPN/PAD-1 family protein	-
PsalSR1_RS23675	45678..45962	-	285	WP_155081858.1	Txe/YoeB family addiction module toxin	Toxin
PsalSR1_RS23680	45966..46226	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PsalSR1_RS23685	46498..46872	+	375	Protein_69	site-specific integrase	-
PsalSR1_RS23690	46929..47153	+	225	WP_155081859.1	transposase	-
PsalSR1_RS23695	47335..47643	+	309	WP_155079958.1	hypothetical protein	-
PsalSR1_RS23700	47640..47990	+	351	WP_155079959.1	IS66 family insertion sequence element accessory protein TnpB	-
PsalSR1_RS23705	48033..49514	+	1482	WP_155080463.1	IS66 family transposase	-
PsalSR1_RS23710	49511..50002	+	492	WP_155081863.1	transposase	-
PsalSR1_RS23715	50158..50706	-	549	WP_155081864.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64745	64745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11193.79 Da
• Isoelectric Point: 8.9660

>T123690 WP_155081858.1 NZ_CP039230:c45962-45678 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHNFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQSLRV

Nucleotide

• Length: 285 bp

>T123690 NZ_CP039230:c45962-45678 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGCCAACGCACGCCGACAGAAGGTATTGGCCATCCCGAACCTTTAAAAC
ATAATTTCAGTGGTTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATCGCTAAGGGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123690 WP_032126795.1 NZ_CP039230:c46226-45966 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123690 NZ_CP039230:c46226-45966 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCGCGGGATAATTTTAAGAGCACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B8U9U2