Detailed information of TA system TA123684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 21294..21907
• Replicon: plasmid unnamed3
• Accession: NZ_CP039222
• Organism: Piscirickettsia salmonis strain NVI 5692

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PsalN5692_RS19215
• Protein ID: WP_155068965.1
• Coordinates: 21584..21907 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PsalN5692_RS19210
• Protein ID: WP_155068964.1
• Coordinates: 21294..21587 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalN5692_RS19190	16614..17786	-	1173	WP_155068961.1	hypothetical protein	-
PsalN5692_RS19195	17931..18332	-	402	WP_155068962.1	hypothetical protein	-
PsalN5692_RS19200	18401..19129	-	729	WP_017375910.1	IS6 family transposase	-
PsalN5692_RS19205	19152..21083	+	1932	WP_155068963.1	hypothetical protein	-
PsalN5692_RS19210	21294..21587	-	294	WP_155068964.1	helix-turn-helix domain-containing protein	Antitoxin
PsalN5692_RS19215	21584..21907	-	324	WP_155068965.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PsalN5692_RS19220	22081..22809	+	729	WP_017375910.1	IS6 family transposase	-
PsalN5692_RS19225	22911..23348	+	438	WP_196215550.1	IS3 family transposase	-
PsalN5692_RS19230	23933..25285	-	1353	WP_196215552.1	DEAD/DEAH box helicase	-
PsalN5692_RS19235	25375..25941	-	567	WP_054300664.1	recombinase family protein	-
PsalN5692_RS19240	25945..26631	-	687	WP_017377658.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..75380	75380
	-	inside	IScluster/Tn	-	-	15883..22809	6926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-90)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12409.66 Da
• Isoelectric Point: 10.5682

>T123684 WP_155068965.1 NZ_CP039222:c21907-21584 [Piscirickettsia salmonis]
MTWRINYFNDKVMEQVLAWPKGVLANYLHISELMQEFGPNLGLPHTKAMSDGLFEIRAKGQEGIGRAFFCTVVRNEVIIL
HSIIKKTQKTPDKDLKLARKRLKELKR

Nucleotide

• Length: 324 bp

>T123684 NZ_CP039222:c21907-21584 [Piscirickettsia salmonis]
ATGACTTGGAGAATCAATTACTTTAATGACAAGGTAATGGAACAAGTCTTAGCATGGCCCAAAGGTGTACTTGCAAACTA
CCTGCATATCAGTGAGTTAATGCAAGAGTTTGGCCCCAACTTAGGTTTGCCCCACACTAAAGCTATGAGTGACGGCTTAT
TTGAAATTAGAGCTAAGGGGCAAGAAGGAATCGGCAGAGCATTTTTTTGTACTGTGGTGAGAAATGAAGTAATTATCCTT
CATTCCATCATTAAAAAAACGCAGAAAACGCCAGATAAAGACTTAAAACTGGCGCGTAAACGATTGAAGGAGTTGAAACG
ATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11052.69 Da
• Isoelectric Point: 9.8109

>AT123684 WP_155068964.1 NZ_CP039222:c21587-21294 [Piscirickettsia salmonis]
MSKRHTHDEFLKKALAKPGVQAIYDDLDEEYQLIRELLLARKQANKSQEEVAQDMHTTRSAISRLESHGKHSPSINTLRK
YAEAVGCKLQIKLVPAK

Nucleotide

• Length: 294 bp

>AT123684 NZ_CP039222:c21587-21294 [Piscirickettsia salmonis]
ATGAGTAAACGACACACTCATGATGAGTTTTTAAAGAAAGCTCTGGCTAAACCAGGGGTTCAAGCTATCTATGATGACTT
GGATGAAGAATACCAGCTTATTCGAGAATTGCTACTCGCTCGCAAACAGGCAAATAAATCTCAAGAAGAGGTTGCTCAAG
ATATGCATACCACGCGCAGTGCAATTTCTCGACTAGAGTCTCATGGTAAACATTCACCCTCTATCAATACATTGCGCAAA
TATGCTGAAGCAGTCGGATGCAAATTGCAAATCAAACTAGTTCCAGCTAAATAG