Detailed information of TA system TA123682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 17639..18175
• Replicon: plasmid unnamed1
• Accession: NZ_CP039220
• Organism: Piscirickettsia salmonis strain NVI 5692

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6I5YBD1
• Locus tag: PsalN5692_RS17790
• Protein ID: WP_016211910.1
• Coordinates: 17639..17911 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: PsalN5692_RS17795
• Protein ID: WP_122943164.1
• Coordinates: 17915..18175 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PsalN5692_RS17760	12943..13194	+	252	WP_196215525.1	hypothetical protein	-
PsalN5692_RS17765	13183..13911	-	729	WP_155068804.1	IS6 family transposase	-
PsalN5692_RS17770	14111..15970	+	1860	WP_155068805.1	ThiF family adenylyltransferase	-
PsalN5692_RS17775	15979..16815	-	837	WP_155066496.1	IS3 family transposase	-
PsalN5692_RS17780	16827..17099	-	273	WP_032126239.1	transposase	-
PsalN5692_RS17785	17118..17618	+	501	WP_196215526.1	Mov34/MPN/PAD-1 family protein	-
PsalN5692_RS17790	17639..17911	-	273	WP_016211910.1	Txe/YoeB family addiction module toxin	Toxin
PsalN5692_RS17795	17915..18175	-	261	WP_122943164.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PsalN5692_RS17800	18666..18851	+	186	WP_155068807.1	hypothetical protein	-
PsalN5692_RS17805	19024..19182	+	159	WP_155068808.1	hypothetical protein	-
PsalN5692_RS17810	19152..20090	+	939	WP_155068809.1	hypothetical protein	-
PsalN5692_RS17815	20087..20514	-	428	Protein_27	RluA family pseudouridine synthase	-
PsalN5692_RS17820	20678..21406	-	729	WP_032126737.1	IS6 family transposase	-
PsalN5692_RS17825	21999..22727	+	729	WP_155068125.1	IS6 family transposase	-
PsalN5692_RS17830	22791..22865	-	75	Protein_30	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137059	137059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10739.22 Da
• Isoelectric Point: 7.8926

>T123682 WP_016211910.1 NZ_CP039220:c17911-17639 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Nucleotide

• Length: 273 bp

>T123682 NZ_CP039220:c17911-17639 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGCCAACGCACGCCGACAGAAGGTATTGGCCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGTTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGACGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9536.89 Da
• Isoelectric Point: 5.1004

>AT123682 WP_122943164.1 NZ_CP039220:c18175-17915 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
HDIIEE

Nucleotide

• Length: 261 bp

>AT123682 NZ_CP039220:c18175-17915 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCGCGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CATGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6I5YBD1