Detailed information of TA system TA123624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1540929..1541352
• Replicon: chromosome
• Accession: NZ_CP039167
• Organism: Staphylococcus aureus strain R50

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E1952_RS07735
• Protein ID: WP_001837494.1
• Coordinates: 1541101..1541352 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: E1952_RS07730
• Protein ID: WP_000028422.1
• Coordinates: 1540929..1541111 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1952_RS07680	1536072..1537733	-	1662	WP_000625088.1	terminase large subunit	-
E1952_RS07685	1537730..1538074	-	345	WP_000402904.1	hypothetical protein	-
E1952_RS07690	1538205..1538504	-	300	WP_000988336.1	HNH endonuclease	-
E1952_RS07695	1538736..1539152	-	417	WP_000590122.1	hypothetical protein	-
E1952_RS07700	1539180..1539380	-	201	WP_000265042.1	DUF1514 family protein	-
E1952_RS07705	1539380..1539529	-	150	WP_000595265.1	transcriptional activator RinB	-
E1952_RS07710	1539526..1539912	-	387	WP_000592207.1	hypothetical protein	-
E1952_RS07715	1539909..1540115	-	207	WP_000195803.1	DUF1381 domain-containing protein	-
E1952_RS07720	1540112..1540357	-	246	WP_001282074.1	hypothetical protein	-
E1952_RS07725	1540394..1540936	-	543	WP_000185660.1	dUTP pyrophosphatase	-
E1952_RS07730	1540929..1541111	-	183	WP_000028422.1	hypothetical protein	Antitoxin
E1952_RS07735	1541101..1541352	-	252	WP_001837494.1	DUF1024 family protein	Toxin
E1952_RS07740	1541345..1541629	-	285	WP_001105620.1	hypothetical protein	-
E1952_RS07750	1541823..1541936	-	114	Protein_1487	hypothetical protein	-
E1952_RS07755	1541937..1542122	-	186	WP_001187245.1	DUF3113 family protein	-
E1952_RS07760	1542127..1542531	-	405	WP_000049795.1	DUF1064 domain-containing protein	-
E1952_RS07765	1542541..1542762	-	222	WP_001123695.1	DUF3269 family protein	-
E1952_RS07770	1542776..1542934	-	159	WP_000256597.1	hypothetical protein	-
E1952_RS07775	1542928..1543707	-	780	WP_000803063.1	ATP-binding protein	-
E1952_RS07780	1543717..1544487	-	771	WP_000190254.1	conserved phage C-terminal domain-containing protein	-
E1952_RS07785	1544553..1544834	+	282	WP_000414755.1	hypothetical protein	-
E1952_RS14815	1544827..1544976	-	150	WP_001081076.1	hypothetical protein	-
E1952_RS07790	1544973..1545644	-	672	WP_001199422.1	hypothetical protein	-
E1952_RS07795	1545657..1546208	-	552	WP_000704705.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	lukF-PV / lukS-PV / sea / gnd	1512764..1578737	65973

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9350.20 Da
• Isoelectric Point: 3.9022

>T123624 WP_001837494.1 NZ_CP039167:c1541352-1541101 [Staphylococcus aureus]
MDNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T123624 NZ_CP039167:c1541352-1541101 [Staphylococcus aureus]
ATGGATAACCGTGAACAAATAGAACAATCAGTGATCAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTGCTAAA
AGAGATTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT123624 WP_000028422.1 NZ_CP039167:c1541111-1540929 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT123624 NZ_CP039167:c1541111-1540929 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VQM8