Detailed information of TA system TA123557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3425..4076
• Replicon: plasmid p10FS3-1-10
• Accession: NZ_CP039153
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: E5Y90_RS17255
• Protein ID: WP_000269911.1
• Coordinates: 3425..3781 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5Y90_RS17260
• Protein ID: WP_001140620.1
• Coordinates: 3774..4076 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS17230	1..951	+	951	WP_002011663.1	replication initiation protein RepM	-
E5Y90_RS17235	944..1519	+	576	WP_002011656.1	helix-turn-helix domain-containing protein	-
E5Y90_RS17240	1540..1683	+	144	WP_001125247.1	hypothetical protein	-
E5Y90_RS17245	1941..2285	+	345	WP_005146112.1	hypothetical protein	-
E5Y90_RS17250	2286..2843	+	558	WP_005146114.1	TetR/AcrR family transcriptional regulator	-
E5Y90_RS17255	3425..3781	+	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5Y90_RS17260	3774..4076	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
E5Y90_RS17265	4150..4647	-	498	WP_174660737.1	hypothetical protein	-
E5Y90_RS17270	4640..5971	-	1332	WP_174660738.1	ATP-binding protein	-
E5Y90_RS17275	6117..6482	-	366	WP_174660739.1	hypothetical protein	-
E5Y90_RS17280	6780..8504	+	1725	WP_174660740.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9137	9137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T123557 WP_000269911.1 NZ_CP039153:3425-3781 [Acinetobacter sp. 10FS3-1]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T123557 NZ_CP039153:3425-3781 [Acinetobacter sp. 10FS3-1]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAATCGACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTTTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT123557 WP_001140620.1 NZ_CP039153:3774-4076 [Acinetobacter sp. 10FS3-1]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT123557 NZ_CP039153:3774-4076 [Acinetobacter sp. 10FS3-1]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3