123553

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 16349..17042 Replicon plasmid p10FS3-1-3
Accession NZ_CP039146
Organism Acinetobacter sp. 10FS3-1

Toxin (Protein)


Gene name higB Uniprot ID R9B971
Locus tag E5Y90_RS16340 Protein ID WP_016165531.1
Coordinates 16752..17042 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6N0LK42
Locus tag E5Y90_RS16335 Protein ID WP_035268402.1
Coordinates 16349..16750 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Y90_RS16310 11731..12661 - 931 Protein_13 IS5 family transposase -
E5Y90_RS16315 12863..14197 + 1335 WP_015060273.1 ISNCY family transposase -
E5Y90_RS16320 14364..15053 + 690 Protein_15 ISNCY family transposase -
E5Y90_RS16325 15051..15647 + 597 Protein_16 IS66 family transposase -
E5Y90_RS16330 15662..16210 - 549 Protein_17 IS3 family transposase -
E5Y90_RS16335 16349..16750 - 402 WP_035268402.1 helix-turn-helix domain-containing protein Antitoxin
E5Y90_RS16340 16752..17042 - 291 WP_016165531.1 type II toxin-antitoxin system HigB family toxin Toxin
E5Y90_RS16345 17208..17462 + 255 Protein_20 transposase -
E5Y90_RS16350 17513..18058 - 546 WP_127800924.1 DNA-binding protein -
E5Y90_RS16355 18215..20050 - 1836 Protein_22 Tn3 family transposase -
E5Y90_RS16360 20114..20818 + 705 WP_174660683.1 IS6-like element IS26 family transposase -
E5Y90_RS16365 20873..21928 - 1056 Protein_24 DUF4158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / sul2 - 1..73803 73803
- inside IScluster/Tn aph(3'')-Ib / aph(6)-Id - 11731..28942 17211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(19-90)

Antitoxin

Antitoxin HigA

(11-133)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11257.12 Da        Isoelectric Point: 10.1259

>T123553 WP_016165531.1 NZ_CP039146:c17042-16752 [Acinetobacter sp. 10FS3-1]
MHVITHARIIEAMHKWPQAETALDGWYRTIKANDPKDFAEMKQLFPAVDKVGKFHVFDIGGNKIRLIAVVMYQAKRVYIR
HVLSHKEYDKGHWKEG

Download         Length: 291 bp

>T123553 NZ_CP039146:c17042-16752 [Acinetobacter sp. 10FS3-1]
ATGCATGTGATCACCCACGCTAGAATTATAGAAGCAATGCACAAGTGGCCACAGGCTGAAACGGCATTAGATGGTTGGTA
TCGAACCATAAAGGCAAACGATCCGAAAGATTTTGCTGAGATGAAACAGCTCTTTCCAGCTGTCGATAAAGTTGGAAAGT
TCCATGTATTCGATATTGGCGGGAACAAGATCCGACTTATTGCCGTCGTGATGTATCAAGCAAAACGTGTGTATATTCGT
CATGTCTTGTCCCATAAAGAATATGACAAAGGTCATTGGAAGGAAGGTTAG

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14823.65 Da        Isoelectric Point: 4.3676

>AT123553 WP_035268402.1 NZ_CP039146:c16750-16349 [Acinetobacter sp. 10FS3-1]
MNAMIKQAVDHWHYVAPLLSKPENEDDFHTLVEALDELLDTVGDDESNPLMGLIHQLGDLVSDYENEHLPMPKGDGRAAL
AFLMEQHGLGQSDLAEVATQSVISEILSGKRQLNIRHIKALSERFNVSADTFF

Download         Length: 402 bp

>AT123553 NZ_CP039146:c16750-16349 [Acinetobacter sp. 10FS3-1]
ATGAACGCGATGATTAAACAGGCCGTAGACCACTGGCACTACGTTGCACCATTGCTCAGCAAGCCAGAAAATGAAGACGA
CTTTCATACACTTGTTGAAGCTTTAGATGAACTTTTAGATACCGTTGGTGATGACGAATCTAATCCTTTAATGGGGCTTA
TTCATCAGCTTGGTGATTTGGTTTCTGATTATGAAAATGAGCATCTTCCTATGCCTAAAGGGGATGGTCGTGCTGCTTTG
GCTTTTTTGATGGAGCAACATGGTTTAGGTCAGTCTGACTTAGCAGAAGTTGCAACCCAATCTGTGATTTCTGAGATTTT
AAGTGGTAAACGCCAACTGAATATTCGTCATATTAAGGCATTGTCTGAGCGGTTTAATGTATCGGCAGATACGTTTTTCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9B971

Antitoxin

Source ID Structure
AlphaFold DB A0A6N0LK42

References