Detailed information of TA system TA123552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 69874..70525
• Replicon: plasmid p10FS3-1-2
• Accession: NZ_CP039145
• Organism: Acinetobacter sp. 10FS3-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: E5Y90_RS16215
• Protein ID: WP_000269911.1
• Coordinates: 70169..70525 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5Y90_RS16210
• Protein ID: WP_001140620.1
• Coordinates: 69874..70176 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y90_RS16190	65092..66675	+	1584	WP_174660671.1	Fic family protein	-
E5Y90_RS16195	67376..68080	-	705	WP_174660672.1	IS6-like element IS1006 family transposase	-
E5Y90_RS16200	68140..68556	-	417	Protein_49	IS5-like element ISAba12 family transposase	-
E5Y90_RS16205	68839..69657	+	819	WP_174660673.1	hypothetical protein	-
E5Y90_RS16210	69874..70176	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
E5Y90_RS16215	70169..70525	-	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5Y90_RS16220	71055..71954	-	900	WP_174660674.1	hypothetical protein	-
E5Y90_RS16225	72481..72651	-	171	WP_174660675.1	helix-turn-helix domain-containing protein	-
E5Y90_RS16230	72657..72755	+	99	Protein_55	IS982 family transposase	-
E5Y90_RS16235	73164..73658	+	495	WP_174660676.1	spore coat protein U domain-containing protein	-
E5Y90_RS16240	73686..74381	+	696	WP_174660677.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..75189	75189

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T123552 WP_000269911.1 NZ_CP039145:c70525-70169 [Acinetobacter sp. 10FS3-1]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T123552 NZ_CP039145:c70525-70169 [Acinetobacter sp. 10FS3-1]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAATCGACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTTTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT123552 WP_001140620.1 NZ_CP039145:c70176-69874 [Acinetobacter sp. 10FS3-1]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT123552 NZ_CP039145:c70176-69874 [Acinetobacter sp. 10FS3-1]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3