Detailed information of TA system TA123544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1338898..1339484
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: E5221_RS06260
• Protein ID: WP_082810539.1
• Coordinates: 1339206..1339484 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: A0A3E1UUV1
• Locus tag: E5221_RS06255
• Protein ID: WP_004375387.1
• Coordinates: 1338898..1339197 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS06230 (E5221_06430)	1334704..1335513	+	810	WP_162276773.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
E5221_RS06235 (E5221_06435)	1335625..1336035	-	411	WP_061550983.1	SufE family protein	-
E5221_RS06240 (E5221_06440)	1336032..1337237	-	1206	WP_182329137.1	cysteine desulfurase	-
E5221_RS06245 (E5221_06445)	1337358..1338392	-	1035	WP_013971245.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
E5221_RS06250 (E5221_06450)	1338424..1338771	-	348	WP_025337970.1	ArsC family reductase	-
E5221_RS06255 (E5221_06455)	1338898..1339197	-	300	WP_004375387.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
E5221_RS06260 (E5221_06460)	1339206..1339484	-	279	WP_082810539.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5221_RS06265 (E5221_06465)	1339733..1341379	+	1647	WP_013971247.1	Na+/H+ antiporter	-
E5221_RS06270 (E5221_06470)	1341531..1342727	-	1197	WP_013971248.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10526.90 Da
• Isoelectric Point: 7.8928

>T123544 WP_082810539.1 NZ_CP039127:c1339484-1339206 [Pseudomonas sp. A2]
MIRSFSCADTEALFTHGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLEALSGNRAGQHSIRVNDQWRLCFIWTD
NGPINVEIVDYH

Nucleotide

• Length: 279 bp

>T123544 NZ_CP039127:c1339484-1339206 [Pseudomonas sp. A2]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCACTTTTTACTCATGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTTGCGGAACGCAAACTGGCCATGCTCGATGCCGCAACAGAGCTGCGAGACCTTCGGTCGCCACCTGGCAACCGTCTCG
AGGCATTGAGCGGCAACCGTGCAGGCCAGCACAGTATCCGTGTCAATGACCAATGGCGCCTGTGCTTTATCTGGACCGAC
AACGGGCCGATTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10877.49 Da
• Isoelectric Point: 5.6714

>AT123544 WP_004375387.1 NZ_CP039127:c1339197-1338898 [Pseudomonas sp. A2]
MLKNGMRPIHPGEVLREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Nucleotide

• Length: 300 bp

>AT123544 NZ_CP039127:c1339197-1338898 [Pseudomonas sp. A2]
ATGCTCAAGAACGGTATGCGTCCGATTCATCCCGGAGAAGTCCTGCGTGAGGACTTCCAGAAAGAAATGGGTTTCAGCGC
CGCGGCACTGGCCCGGGCGCTAGGCGTTGCCACGCCAACCGTCAACAACATCCTGCGTGAACGGGGCGGTGTTTCCGCCG
ACATGGCGCTGCGCCTGTCCATCTGCCTGGACACCACCCCCGAGTTCTGGCTCAACCTGCAGACCGCCTTCGATCTGCGC
ACCGCCGAACAGCAACACGGTGATGAGATCATCGGTTCGGTACAGCGCCTGGTCGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3E1UUV1