Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 22518..23054 Replicon plasmid unnamed1
Accession NZ_CP039113
Organism Piscirickettsia salmonis strain Psal-135

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag Psal135_RS16285 Protein ID WP_036772441.1
Coordinates 22782..23054 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag Psal135_RS16280 Protein ID WP_032126795.1
Coordinates 22518..22778 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Psal135_RS16245 18184..19161 - 978 WP_036771347.1 IS5 family transposase -
Psal135_RS16250 19236..19886 + 651 WP_155046631.1 hypothetical protein -
Psal135_RS16255 19970..20176 + 207 Protein_25 IS6 family transposase -
Psal135_RS16260 20227..20394 - 168 Protein_26 IS6 family transposase -
Psal135_RS16265 20464..21096 + 633 Protein_27 IS6-like element ISPsa2 family transposase -
Psal135_RS16270 21162..21728 - 567 Protein_28 IS6 family transposase -
Psal135_RS16275 22014..22178 - 165 Protein_29 IS6 family transposase -
Psal135_RS16280 22518..22778 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Psal135_RS16285 22782..23054 + 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
Psal135_RS16290 23130..23858 + 729 WP_017375910.1 IS6 family transposase -
Psal135_RS16295 24428..25492 - 1065 WP_174872554.1 IS91 family transposase -
Psal135_RS18670 25672..25851 - 180 WP_157894752.1 hypothetical protein -
Psal135_RS16300 26264..27091 + 828 WP_048876208.1 IS30 family transposase -
Psal135_RS16305 27134..27863 - 730 Protein_36 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..186021 186021
- inside IScluster/Tn - - 2129..33477 31348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T123535 WP_036772441.1 NZ_CP039113:22782-23054 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T123535 NZ_CP039113:22782-23054 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT123535 WP_032126795.1 NZ_CP039113:22518-22778 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT123535 NZ_CP039113:22518-22778 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References