Detailed information of TA system TA123479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 149533..150069
• Replicon: plasmid unnamed1
• Accession: NZ_CP039061
• Organism: Piscirickettsia salmonis strain Psal-099

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1L6THY5
• Locus tag: Psal099_RS17140
• Protein ID: WP_036772441.1
• Coordinates: 149533..149805 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: Psal099_RS17145
• Protein ID: WP_032126795.1
• Coordinates: 149809..150069 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal099_RS17115	144725..145454	+	730	Protein_188	IS6 family transposase	-
Psal099_RS17120	145497..146324	-	828	WP_048876208.1	IS30 family transposase	-
Psal099_RS18705	146737..146916	+	180	WP_157894752.1	hypothetical protein	-
Psal099_RS17130	147096..148160	+	1065	WP_174872554.1	IS91 family transposase	-
Psal099_RS17135	148729..149457	-	729	WP_017375910.1	IS6 family transposase	-
Psal099_RS17140	149533..149805	-	273	WP_036772441.1	Txe/YoeB family addiction module toxin	Toxin
Psal099_RS17145	149809..150069	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Psal099_RS17150	150409..150573	+	165	Protein_195	IS6 family transposase	-
Psal099_RS17155	150859..151425	+	567	Protein_196	IS6 family transposase	-
Psal099_RS17160	151491..152123	-	633	Protein_197	IS6-like element ISPsa2 family transposase	-
Psal099_RS17165	152193..152360	+	168	Protein_198	IS6 family transposase	-
Psal099_RS17170	152411..152617	-	207	Protein_199	IS6 family transposase	-
Psal099_RS17175	152701..153351	-	651	WP_155046631.1	hypothetical protein	-
Psal099_RS17180	153426..154403	+	978	WP_036771347.1	IS5 family transposase	-
Psal099_RS17185	154418..155023	+	606	WP_155050395.1	sulfite exporter TauE/SafE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..175441	175441
	-	inside	IScluster/Tn	-	-	139111..172212	33101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10785.31 Da
• Isoelectric Point: 7.8926

>T123479 WP_036772441.1 NZ_CP039061:c149805-149533 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Nucleotide

• Length: 273 bp

>T123479 NZ_CP039061:c149805-149533 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123479 WP_032126795.1 NZ_CP039061:c150069-149809 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123479 NZ_CP039061:c150069-149809 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L6THY5

Antitoxin

• AlphaFold DB: A0A0B8U9U2