Detailed information of TA system TA123463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 158456..158992
• Replicon: plasmid unnamed1
• Accession: NZ_CP039047
• Organism: Piscirickettsia salmonis strain Psal-073

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1L6THY5
• Locus tag: Psal073_RS17025
• Protein ID: WP_036772441.1
• Coordinates: 158456..158728 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: Psal073_RS17030
• Protein ID: WP_032126795.1
• Coordinates: 158732..158992 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal073_RS16995	153645..154374	+	730	Protein_187	IS6 family transposase	-
Psal073_RS17000	154417..155244	-	828	WP_048876208.1	IS30 family transposase	-
Psal073_RS17005	155657..155812	+	156	WP_155069811.1	hypothetical protein	-
Psal073_RS17010	155957..156190	+	234	WP_155069813.1	transposase zinc-binding domain-containing protein	-
Psal073_RS17015	156109..157080	+	972	WP_048876229.1	IS91 family transposase	-
Psal073_RS17020	157652..158380	-	729	WP_017375910.1	IS6 family transposase	-
Psal073_RS17025	158456..158728	-	273	WP_036772441.1	Txe/YoeB family addiction module toxin	Toxin
Psal073_RS17030	158732..158992	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Psal073_RS17035	159332..160060	+	729	WP_155069815.1	IS6 family transposase	-
Psal073_RS17040	160126..160758	-	633	Protein_196	IS6-like element ISPsa2 family transposase	-
Psal073_RS17045	160828..160995	+	168	Protein_197	IS6 family transposase	-
Psal073_RS17050	161046..161252	-	207	Protein_198	IS6 family transposase	-
Psal073_RS17055	161336..162151	-	816	WP_036775032.1	hypothetical protein	-
Psal073_RS17060	162199..162888	-	690	WP_017375909.1	hypothetical protein	-
Psal073_RS17065	163177..163905	+	729	WP_155069817.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..208052	208052
	-	inside	IScluster/Tn	-	-	148403..182870	34467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10785.31 Da
• Isoelectric Point: 7.8926

>T123463 WP_036772441.1 NZ_CP039047:c158728-158456 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Nucleotide

• Length: 273 bp

>T123463 NZ_CP039047:c158728-158456 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123463 WP_032126795.1 NZ_CP039047:c158992-158732 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123463 NZ_CP039047:c158992-158732 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L6THY5

Antitoxin

• AlphaFold DB: A0A0B8U9U2