Detailed information of TA system TA123307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4300057..4300659
• Replicon: chromosome
• Accession: NZ_CP038859
• Organism: Escherichia coli strain 357900

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A13_RS20715
• Protein ID: WP_000897305.1
• Coordinates: 4300057..4300368 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A13_RS20720
• Protein ID: WP_000356397.1
• Coordinates: 4300369..4300659 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A13_RS20690	4295971..4296570	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A13_RS20695	4296564..4297436	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A13_RS20700	4297433..4297870	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A7A13_RS20705	4297915..4298856	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A7A13_RS20710	4298920..4299828	-	909	WP_001162704.1	alpha/beta hydrolase	-
A7A13_RS20715	4300057..4300368	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A13_RS20720	4300369..4300659	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A7A13_RS20725	4301264..4301482	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
A7A13_RS20730	4301701..4301943	+	243	WP_001087409.1	hypothetical protein	-
A7A13_RS20735	4302273..4303202	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
A7A13_RS20740	4303199..4303834	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A13_RS20745	4303831..4304733	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T123307 WP_000897305.1 NZ_CP038859:4300057-4300368 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T123307 NZ_CP038859:4300057-4300368 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT123307 WP_000356397.1 NZ_CP038859:4300369-4300659 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT123307 NZ_CP038859:4300369-4300659 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7