Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2171054..2171556 Replicon chromosome
Accession NZ_CP038859
Organism Escherichia coli strain 357900

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A7A13_RS10545 Protein ID WP_000767829.1
Coordinates 2171054..2171308 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A7A13_RS10550 Protein ID WP_001259255.1
Coordinates 2171305..2171556 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A13_RS10515 2166069..2166296 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A7A13_RS10520 2166310..2167368 - 1059 WP_000492301.1 transport protein YeeE -
A7A13_RS10525 2167547..2168905 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A7A13_RS10530 2168895..2168957 - 63 WP_010723108.1 membrane protein YoeI -
A7A13_RS10535 2169172..2170101 - 930 WP_000803351.1 LysR family transcriptional regulator -
A7A13_RS10540 2170147..2170971 - 825 WP_000754758.1 SDR family oxidoreductase -
A7A13_RS10545 2171054..2171308 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A7A13_RS10550 2171305..2171556 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A7A13_RS10555 2171839..2171889 + 51 WP_001364200.1 his operon leader peptide -
A7A13_RS10560 2172035..2172934 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A7A13_RS10565 2172940..2174244 + 1305 WP_024261505.1 histidinol dehydrogenase -
A7A13_RS10570 2174241..2175311 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
A7A13_RS10575 2175311..2176378 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123299 WP_000767829.1 NZ_CP038859:c2171308-2171054 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123299 NZ_CP038859:c2171308-2171054 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123299 WP_001259255.1 NZ_CP038859:c2171556-2171305 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123299 NZ_CP038859:c2171556-2171305 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References