Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 273393..273934 Replicon chromosome
Accession NZ_CP038859
Organism Escherichia coli strain 357900

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag A7A13_RS01225 Protein ID WP_000615982.1
Coordinates 273393..273671 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A13_RS01230 Protein ID WP_000729704.1
Coordinates 273674..273934 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A13_RS01210 270975..271553 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A13_RS01215 271759..272526 + 768 WP_000333380.1 class II glutamine amidotransferase -
A7A13_RS01220 272497..273237 - 741 WP_001225679.1 murein L,D-transpeptidase -
A7A13_RS01225 273393..273671 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A13_RS01230 273674..273934 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A13_RS01235 274120..274893 + 774 WP_000543897.1 C40 family peptidase -
A7A13_RS01240 274950..275306 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A7A13_RS01245 275299..275577 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A13_RS01250 275682..277421 - 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
A7A13_RS01255 277366..278151 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T123283 WP_000615982.1 NZ_CP038859:c273671-273393 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T123283 NZ_CP038859:c273671-273393 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT123283 WP_000729704.1 NZ_CP038859:c273934-273674 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT123283 NZ_CP038859:c273934-273674 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References