Detailed information of TA system TA123280

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91836..92105
• Replicon: plasmid unnamed
• Accession: NZ_CP038858
• Organism: Escherichia coli strain PigCaeca_2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E5285_RS24145
• Protein ID: WP_001312861.1
• Coordinates: 91947..92105 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91836..91901 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5285_RS24560	87442..87648	+	207	WP_096949675.1	single-stranded DNA-binding protein	-
E5285_RS24120	87674..88213	+	540	WP_000290838.1	single-stranded DNA-binding protein	-
E5285_RS24125	88276..88509	+	234	WP_000005990.1	DUF905 family protein	-
E5285_RS24130	88575..90462	+	1888	Protein_95	ParB/RepB/Spo0J family partition protein	-
E5285_RS24135	90517..90951	+	435	WP_096949674.1	conjugation system SOS inhibitor PsiB	-
E5285_RS24140	90948..91667	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
E5285_RS24480	91679..91867	-	189	WP_001299721.1	hypothetical protein	-
-	91679..91903	+	225	NuclAT_0	-	-
-	91679..91903	+	225	NuclAT_0	-	-
-	91679..91903	+	225	NuclAT_0	-	-
-	91679..91903	+	225	NuclAT_0	-	-
-	91836..91901	+	66	-	-	Antitoxin
E5285_RS24145	91947..92105	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
E5285_RS24160	93026..93313	+	288	WP_096949673.1	hypothetical protein	-
E5285_RS24165	93431..94252	+	822	WP_135927465.1	DUF945 domain-containing protein	-
E5285_RS24170	94549..95196	-	648	WP_122998621.1	transglycosylase SLT domain-containing protein	-
E5285_RS24175	95473..95856	+	384	WP_025269854.1	relaxosome protein TraM	-
E5285_RS24180	96047..96733	+	687	WP_122998622.1	PAS domain-containing protein	-
E5285_RS24185	96827..97054	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..131158	131158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T123280 WP_001312861.1 NZ_CP038858:91947-92105 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T123280 NZ_CP038858:91947-92105 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT123280 NZ_CP038858:91836-91901 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin