Detailed information of TA system TA123273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3703422..3704116
• Replicon: chromosome
• Accession: NZ_CP038857
• Organism: Escherichia coli strain PigCaeca_2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: E5285_RS18100
• Protein ID: WP_001263493.1
• Coordinates: 3703422..3703820 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E5285_RS18105
• Protein ID: WP_000554757.1
• Coordinates: 3703823..3704116 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5285_RS18070	3698422..3699666	-	1245	WP_000189541.1	esterase FrsA	-
-	3699082..3699162	-	81	NuclAT_10	-	-
-	3699082..3699162	-	81	NuclAT_10	-	-
-	3699082..3699162	-	81	NuclAT_10	-	-
-	3699082..3699162	-	81	NuclAT_10	-	-
E5285_RS18075	3699758..3700216	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E5285_RS18080	3700477..3701934	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
E5285_RS18085	3701991..3702512	-	522	Protein_3506	peptide chain release factor H	-
E5285_RS18090	3702511..3702714	-	204	Protein_3507	RNA ligase RtcB family protein	-
E5285_RS18095	3702960..3703412	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
E5285_RS18100	3703422..3703820	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E5285_RS18105	3703823..3704116	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E5285_RS18110	3704168..3705223	-	1056	WP_001226164.1	DNA polymerase IV	-
E5285_RS18115	3705294..3706217	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
E5285_RS18120	3706220..3707083	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
E5285_RS18125	3707096..3707812	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
E5285_RS18130	3707832..3708299	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T123273 WP_001263493.1 NZ_CP038857:c3703820-3703422 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T123273 NZ_CP038857:c3703820-3703422 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT123273 WP_000554757.1 NZ_CP038857:c3704116-3703823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT123273 NZ_CP038857:c3704116-3703823 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6