Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1805065..1805567 Replicon chromosome
Accession NZ_CP038857
Organism Escherichia coli strain PigCaeca_2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E5285_RS08570 Protein ID WP_000767829.1
Coordinates 1805313..1805567 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E5285_RS08565 Protein ID WP_001259255.1
Coordinates 1805065..1805316 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5285_RS08540 1800242..1801309 - 1068 WP_000080133.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E5285_RS08545 1801309..1802379 - 1071 WP_001099215.1 histidinol-phosphate transaminase -
E5285_RS08550 1802376..1803680 - 1305 WP_135927280.1 histidinol dehydrogenase -
E5285_RS08555 1803686..1804585 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E5285_RS08560 1804731..1804781 - 51 WP_001364200.1 his operon leader peptide -
E5285_RS08565 1805065..1805316 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E5285_RS08570 1805313..1805567 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E5285_RS08575 1805650..1806474 + 825 WP_000754762.1 SDR family oxidoreductase -
E5285_RS08580 1806520..1807449 + 930 WP_000803360.1 LysR family transcriptional regulator -
E5285_RS08585 1807664..1807726 + 63 WP_010723108.1 membrane protein YoeI -
E5285_RS08590 1807716..1809074 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E5285_RS08595 1809253..1810311 + 1059 WP_000492305.1 transport protein YeeE -
E5285_RS08600 1810325..1810552 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123260 WP_000767829.1 NZ_CP038857:1805313-1805567 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123260 NZ_CP038857:1805313-1805567 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123260 WP_001259255.1 NZ_CP038857:1805065-1805316 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123260 NZ_CP038857:1805065-1805316 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References