Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2359973..2360535 Replicon chromosome
Accession NZ_CP038856
Organism Citrobacter freundii strain Upstream_1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag E5284_RS11180 Protein ID WP_096757033.1
Coordinates 2360257..2360535 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E5284_RS11175 Protein ID WP_096757032.1
Coordinates 2359973..2360257 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5284_RS11160 2355274..2358321 + 3048 WP_117342799.1 formate dehydrogenase-N subunit alpha -
E5284_RS11165 2358335..2359219 + 885 WP_115258486.1 formate dehydrogenase N subunit beta -
E5284_RS11170 2359212..2359868 + 657 WP_096757031.1 formate dehydrogenase-N subunit gamma -
E5284_RS11175 2359973..2360257 - 285 WP_096757032.1 HigA family addiction module antidote protein Antitoxin
E5284_RS11180 2360257..2360535 - 279 WP_096757033.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E5284_RS11185 2360723..2362438 - 1716 WP_135912032.1 ATP-binding cassette domain-containing protein -
E5284_RS11190 2362749..2364446 - 1698 WP_115258488.1 malate dehydrogenase -
E5284_RS11195 2364626..2364766 - 141 WP_096757036.1 stationary-phase-induced ribosome-associated protein -
E5284_RS11200 2364995..2365426 + 432 WP_096757037.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10601.05 Da        Isoelectric Point: 9.5320

>T123250 WP_096757033.1 NZ_CP038856:c2360535-2360257 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T123250 NZ_CP038856:c2360535-2360257 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGCGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTAACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTTCAGGCAACTGGCGTATTACGTTTGAATTCGTTAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10327.05 Da        Isoelectric Point: 8.8094

>AT123250 WP_096757032.1 NZ_CP038856:c2360257-2359973 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Download         Length: 285 bp

>AT123250 NZ_CP038856:c2360257-2359973 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References