Detailed information of TA system TA123240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 318153..318819
• Replicon: chromosome
• Accession: NZ_CP038856
• Organism: Citrobacter freundii strain Upstream_1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E5284_RS01510
• Protein ID: WP_117342464.1
• Coordinates: 318502..318819 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: E5284_RS01505
• Protein ID: WP_003837894.1
• Coordinates: 318153..318449 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5284_RS01490	315329..315802	-	474	WP_003023524.1	transcription elongation factor GreB	-
E5284_RS01495	316029..316748	+	720	WP_001157751.1	two-component system response regulator OmpR	-
E5284_RS01500	316745..318097	+	1353	WP_135911287.1	two-component system sensor histidine kinase EnvZ	-
E5284_RS01505	318153..318449	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
E5284_RS01510	318502..318819	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5284_RS01515	318943..320565	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
E5284_RS01520	320944..322662	+	1719	WP_135911288.1	DUF4153 domain-containing protein	-
E5284_RS01525	322713..323591	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T123240 WP_117342464.1 NZ_CP038856:c318819-318502 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T123240 NZ_CP038856:c318819-318502 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAGGGATTTAGTAAACGCCGTCCGTTACTTTTACCTGACGATGAGTTTCGTGCCTTTCA
AGAGGCGCTTATTGAAAACCCTGAGGCAGGGGATACTATCGCTGGTACGGGGGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGTAAGCGCAGCGGTATTCGGGTTATCTATTACAACGTGACGCGTAAAGGTCGAATCTATCTGGCTTTGCTC
TATCCTAAGAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCGATATGCTGATTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT123240 WP_003837894.1 NZ_CP038856:c318449-318153 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT123240 NZ_CP038856:c318449-318153 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTACGTATAGAGAAAGGTGAAGAAACACCTAA
ACCTGAACATGTGCATACTTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCGGTTGGTGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAACCCACTGGAGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAACCCATTTATAATTAATCAATTAAAAGCAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5