123235

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 175203..175927 Replicon plasmid pVag1
Accession NZ_CP038854
Organism Pantoea vagans strain LMG 24199

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EGO56_RS19720 Protein ID WP_135910751.1
Coordinates 175203..175514 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EGO56_RS19725 Protein ID WP_135910752.1
Coordinates 175511..175927 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGO56_RS19700 170494..171600 + 1107 WP_135911031.1 site-specific integrase -
EGO56_RS19705 171618..172190 - 573 WP_135910748.1 lipid IV(A) palmitoyltransferase PagP -
EGO56_RS19715 172634..174856 + 2223 WP_135910750.1 ferric-rhodotorulic acid/ferric-coprogen receptor FhuE -
EGO56_RS19720 175203..175514 + 312 WP_135910751.1 type II toxin-antitoxin system HigB family toxin Toxin
EGO56_RS19725 175511..175927 + 417 WP_135910752.1 helix-turn-helix domain-containing protein Antitoxin
EGO56_RS19730 176038..176883 - 846 WP_135910753.1 VOC family protein -
EGO56_RS19735 176904..178043 - 1140 WP_135910754.1 amidohydrolase -
EGO56_RS19740 178046..179146 - 1101 WP_135910755.1 alkylhydroperoxidase domain protein -
EGO56_RS19745 179143..180150 - 1008 WP_135910756.1 putative FMN-dependent luciferase-like monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla / vipA/tssB / vipB/tssC / vasE/tssK / dotU/tssL / hcp/tssD / clpV/tssH / vgrG/tssI / KPHS_23120 / icmF/tssM / tssF / tssG / sciN/tssJ / iroN 1..559692 559692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12227.13 Da        Isoelectric Point: 9.4339

>T123235 WP_135910751.1 NZ_CP038854:175203-175514 [Pantoea vagans]
MHVISRLPFDEAARKHPNAAAALDLLYRHLRQGDFSCPDDLRALYASLDRMKYRAKWWVIDVSGNQLRVIFFADFERSKI
FIKHICTHAEYDKLVKKYREVTE

Download         Length: 312 bp

>T123235 NZ_CP038854:175203-175514 [Pantoea vagans]
ATGCATGTTATTTCACGCTTGCCGTTTGACGAGGCGGCCAGAAAACATCCCAATGCAGCGGCAGCACTGGATCTGCTTTA
CCGCCACCTCAGACAGGGCGATTTCAGTTGTCCTGACGATCTCAGGGCGCTTTATGCAAGCCTTGATCGTATGAAGTATC
GCGCTAAATGGTGGGTCATTGACGTCAGTGGCAACCAGTTGCGCGTGATATTTTTCGCGGATTTTGAGCGCAGCAAAATC
TTCATCAAACATATCTGCACTCACGCTGAGTACGACAAGCTTGTTAAGAAATATCGGGAGGTAACTGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15526.58 Da        Isoelectric Point: 4.6685

>AT123235 WP_135910752.1 NZ_CP038854:175511-175927 [Pantoea vagans]
MNTEAIRLSNQLAEAVPLLAGSNQRHDYEEALELVEYLIEHEPDNPLVDLLCARIAHYEDNDPAMADFNSRQREISTGPG
VLRVLIEQHNLSLSDFENEIGKKSLVSRILSGERQLTVSHIRALCARFGLSPSLFFLI

Download         Length: 417 bp

>AT123235 NZ_CP038854:175511-175927 [Pantoea vagans]
ATGAATACTGAAGCTATCAGGCTGTCAAATCAGCTGGCAGAGGCGGTGCCGCTGCTGGCCGGCAGCAACCAGCGACATGA
TTATGAAGAAGCACTGGAACTGGTCGAATACCTTATTGAACATGAGCCGGATAATCCGCTGGTAGATTTGCTTTGTGCCC
GCATTGCTCATTATGAAGATAATGATCCTGCGATGGCCGACTTTAATAGCCGCCAGCGTGAAATCAGCACCGGGCCTGGC
GTTCTGAGGGTACTGATCGAGCAGCACAACCTCAGCCTGAGTGACTTCGAAAATGAAATTGGCAAAAAGTCGCTGGTCAG
CCGAATCCTCAGCGGCGAACGTCAGTTGACCGTGTCACACATACGGGCATTGTGCGCGCGGTTTGGCCTGTCACCATCGT
TGTTTTTTTTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References